ArticleGlycobiology2019
Direct fluorescent glycan labeling with recombinant sialyltransferases.
Article in Glycobiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Bidirectional signals generated by Siglec-7 and its crucial ligand tri-sialylated T to escape of cancer cells from immune surveillance.iScience · 2024Article
- Endoglycosidase assay using enzymatically synthesized fluorophore-labeled glycans as substrates to uncover enzyme substrate specificities.Communications biology · 2022Article
- Article
- Direct N- or C-Terminal Protein Labeling Via a Sortase-Mediated Swapping Approach.Bioconjugate chemistry · 2021Article
- Fluorescent glycan fingerprinting of SARS2 spike proteins.Scientific reports · 2021Article
- Cellular and Molecular Engineering of Glycan Sialylation in Heterologous Systems.Molecules (Basel, Switzerland) · 2021Review
- Genome-wide CRISPR screens reveal a specific ligand for the glycan-binding immune checkpoint receptor Siglec-7.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Detecting substrate glycans of fucosyltransferases with fluorophore-conjugated fucose and methods for glycan electrophoresis.Glycobiology · 2020Article
- Fluorescent Detection ofBioconjugate chemistry · 2020Article
- Site-specific N-glycosylation of HeLa cell glycoproteins.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and detection of N- and O-glycans on glycoproteins. The method allows detection of specific glycans without the laborious gel blotting and chemiluminescence reactions used in Western blotting. The method can also be used with a variety of fluorescent dyes.
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Registered trials
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