Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation.

Tatiana V Lapina, Lidiya Yu Kochemasova, Karl Forchhammer, Elena V Ermilova

Abstract read

In one paragraph

Article in Planta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 52% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

Arginine-Dependent Nitric Oxide Generation and S-Nitrosation in the Non-Photosynthetic Unicellular AlgaAntioxidants (Basel, Switzerland) · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 2 countries.

Tatiana V LapinaBiological Faculty, Saint-Petersburg State University, Universitetskaya nab. 7/9, Saint-Petersburg, Russia, 199034.ORCID http://orcid.org/0000-0003-0800-5933

Lidiya Yu KochemasovaBiological Faculty, Saint-Petersburg State University, Universitetskaya nab. 7/9, Saint-Petersburg, Russia, 199034.

Karl ForchhammerOrganismic Interactions Department, Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Eberhard-Karls-Universität Tübingen, Auf der Morgenstelle 28, 72076, Tübingen, Germany.

Elena V ErmilovaBiological Faculty, Saint-Petersburg State University, Universitetskaya nab. 7/9, Saint-Petersburg, Russia, 199034. e.ermilova@spbu.ru.

St Petersburg University · RUUniversity of Tübingen · DE

Funding

DFG Fo195/13-1DFG Fo195/9-2Russian Science Foundation 16-14-10004-ПSaint-Petersburg State University Grant 1.65.38.2017

6 · The paper itself

Abstract

MAIN

conclusionL-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that L-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

Indexed as

Algal ProteinsArginineChlorophyceaeLipid DropletsNitrogenPhosphotransferases (Carboxyl Group Acceptor)PII Nitrogen Regulatory ProteinsSignal TransductionTriglyceridesacetylglutamate kinaseAlgal ProteinsArginineNitrogenPhosphotransferases (Carboxyl Group Acceptor)PII Nitrogen Regulatory ProteinsTriglyceridesGLB-encoded signaling proteinNitrogenNonphotosynthetic green algaeOil droplets

Identifiers

PMID31359139

DOI10.1007/s00425-019-03249-5

OpenAlexW2966118946

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.