ArticleStem cell research & therapy2019
High temperature suppressed SSC self-renewal through S phase cell cycle arrest but not apoptosis.
Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- Provoking Potent Cellular Ferroptosis in the Thermo-Sensitive S-Phase via CPT-11 Loaded Janus Nanomedicine for Enhanced Cancer Therapy.Advanced healthcare materials · 2026Article
- Mitochondria-Targeting microRNAs (mitomiRs): Potential Mediators of Environmental Mitoepigenetics in Mammalian Spermatogenesis.Biomolecules · 2026Review
- A Follow-Up Study on Testicular Cellular and Transcriptomic Responses to Mild Scrotal Heat Stress in Wugu-Hu and Hu Rams.Animals : an open access journal from MDPI · 2026Article
- The effect of salubrinal on the endoplasmic reticulum stress pathway in heat-stressed spermatogonial cells in vitro.FEBS open bio · 2026Article
- Spermatogonial Stem Cells in Domestic Animals: Current Insights and Future Directions with a Focus on Dogs.Veterinary sciences · 2025Review
- Role of NTRK Fusion Genes in the Tumor Immune Microenvironment of HPV (+/-) Cervical Cancer.Journal of medical virology · 2025Review
- Abnormal H3K27me3 underlies degenerative spermatogonial stem cells in cryptorchid testis.Development (Cambridge, England) · 2025Article
- Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling.Cell reports. Medicine · 2024Article
- Deficiency of ASGR1 Alleviates Diet-Induced Systemic Insulin Resistance via Improved Hepatic Insulin Sensitivity.Diabetes & metabolism journal · 2024Article
- Heat stress and stallion fertility.Journal of animal science and technology · 2023Review
- Transcriptome Analysis in High Temperature Inhibiting Spermatogonial Stem Cell Differentiation In Vitro.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Potential Function of Testicular MicroRNAs in Heat-Stress-Induced Spermatogenesis Disorders.International journal of molecular sciences · 2023Article
- Favorable culture conditions for spermatogonial propagation in human and non-human primate primary testicular cell cultures: a systematic review and meta-analysis.Frontiers in cell and developmental biology · 2023Article
- Nicotinamide mononucleotide improves spermatogenic function in streptozotocin-induced diabetic mice via modulating the glycolysis pathway.Acta biochimica et biophysica Sinica · 2022Article
- Impact of Temperature and Time Interval Prior to Immature Testicular-Tissue Organotypic Culture on Cellular Niche.Reproductive sciences (Thousand Oaks, Calif.) · 2021Article
- Responses and coping methods of different testicular cell types to heat stress: overview and perspectives.Bioscience reports · 2021Review
- Sox2 Localization During Spermatogenesis and Its Association with other Spermatogenesis Markers Using Protein-Protein Network Analysis.Journal of reproduction & infertilityArticle
- Reversibility of Spermatogonial Stem Cell Injury After Single Acute Scrotal Hyperthermia in Mouse and Rat Models: A Systematic Review.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh temperature has a very adverse effect on mammalian spermatogenesis and eventually leads to sub- or infertility through either apoptosis or DNA damage. However, the direct effects of heat stress on the development of spermatogonial stem cells (SSCs) are still unknown because SSCs are rare in the testes.
methodsIn the present study, we first used in vitro-cultured SSCs to study the effect of heat shock treatment on SSC development. Then, we used RNA-Seq analysis to identify new genes or signalling pathways implicated in the heat stress response.
resultsWe found that 45 min of 43 °C heat shock treatment significantly inhibited the proliferation of SSCs 2 h after treatment but did not lead to apoptosis. In total, 17,822 genes were identified by RNA-Seq after SSC heat shock treatment. Among these genes, we found that 200 of them had significantly changed expression, with 173 upregulated and 27 downregulated genes. The number of differentially expressed genes in environmental information processing pathways was 37, which was the largest number. We screened the candidate JAK-STAT signalling pathway on the basis of inhibition of cell cycle progression and found that the JAK-STAT pathway was inhibited after heat shock treatment. The flow cytometry results further confirmed that heat stress caused S phase cycle arrest of SSCs.
conclusionOur results showed that heat shock treatment at 43 °C for 45 min significantly inhibited SSC self-renewal through S phase cell cycle arrest but not apoptosis.
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