Evidence map›Paper›PMID 31358052›Full record

ArticleStem cell research & therapy2019

The role of insulin as a key regulator of seeding, proliferation, and mRNA transcription of human pluripotent stem cells.

Mohammad Shahbazi, Paige Cundiff, Wenyu Zhou, Philip Lee, Achchhe Patel, Sunita L D'Souza, Fahim Abbasi, Thomas Quertermous, Joshua W Knowles

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Insulin and cancer: a tangled web.The Biochemical journal · 2022
    Review
  4. Article
  5. Review
  6. PI3K in stemness regulation: from development to cancer.Biochemical Society transactions · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Mohammad ShahbaziStanford Cardiovascular Medicine and Cardiovascular Institute, Stanford School of Medicine, Stanford University, Falk CVRC, Room CV273, MC 5406 300 Pasteur Drive, Stanford, CA, 94305, USA.
Paige CundiffDepartment of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, Mount Sinai, New York, NY, 10029, USA.
Wenyu ZhouStanford Center for Genomics and Personalized Medicine, Stanford University, Stanford, CA, 94305, USA.
Philip LeeStanford Cardiovascular Medicine and Cardiovascular Institute, Stanford School of Medicine, Stanford University, Falk CVRC, Room CV273, MC 5406 300 Pasteur Drive, Stanford, CA, 94305, USA.
Achchhe PatelDepartment of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, Mount Sinai, New York, NY, 10029, USA.
Sunita L D'SouzaDepartment of Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, Mount Sinai, New York, NY, 10029, USA.
Fahim AbbasiStanford Cardiovascular Medicine and Cardiovascular Institute, Stanford School of Medicine, Stanford University, Falk CVRC, Room CV273, MC 5406 300 Pasteur Drive, Stanford, CA, 94305, USA.
Thomas QuertermousStanford Cardiovascular Medicine and Cardiovascular Institute, Stanford School of Medicine, Stanford University, Falk CVRC, Room CV273, MC 5406 300 Pasteur Drive, Stanford, CA, 94305, USA.
Joshua W KnowlesStanford Cardiovascular Medicine and Cardiovascular Institute, Stanford School of Medicine, Stanford University, Falk CVRC, Room CV273, MC 5406 300 Pasteur Drive, Stanford, CA, 94305, USA. knowlej@stanford.edu.ORCID 0000-0003-1922-7240
Cardiovascular Institute of the South · USIcahn School of Medicine at Mount Sinai · USStanford University · US

Funding

Stanford Islet Research CoreP30DK116074 · NIDDK · STANFORD UNIVERSITY · PI Seung K Kim · 2017 to 2026
$19.5M
Identifying the gene networks of insulin resistance: the GENESIPS studyU01HL107388 · NHLBI · STANFORD UNIVERSITY · PI QUERTERMOUS, THOMAS, SCHADT, ERIC E · 2011 to 2015
$8.8M
Characterization of novel insulin resistance genes by gene editing, high-throughput phenotyping and in vivo studiesR01DK120565 · NIDDK · STANFORD UNIVERSITY · PI KNOWLES, JOSHUA WILEY · 2019 to 2023
$3.2M
Mechanisms of NAT2 Regulation of Insulin Resistance and Mitochondrial DysfunctionR01DK116750 · NIDDK · STANFORD UNIVERSITY · PI KNOWLES, JOSHUA WILEY · 2019 to 2023
$2.4M
Beyond GWAS of insulin resistance: An integrated approach to translate genetic association to functionR01DK106236 · NIDDK · STANFORD UNIVERSITY · PI KNOWLES, JOSHUA WILEY · 2016 to 2020
$2.4M
Doris Duke Charitable Foundation 2016097NIDDK NIH HHS P30 DK116074NIDDK NIH HHS R01 DK106236NIDDK NIH HHS R01 DK116750NIDDK NIH HHS R01 DK120565NIH HHS U01HL107388
6 · The paper itself

Abstract

backgroundHuman-induced pluripotent stem cells (hiPSCs) show a great promise as a renewable source of cells with broad biomedical applications. Since insulin has been used in the maintenance of hiPSCs, in this study we explored the role of insulin in culture of these cells.

methodsWe report conditions for insulin starvation and stimulation of hiPSCs. Crystal violet staining was used to study the adhesion and proliferation of hiPSCs. Apoptosis and cell cycle assays were performed through flow cytometry. Protein arrays were used to confirm phosphorylation targets, and mRNA sequencing was used to evaluate the effect of transcriptome.

resultsInsulin improved the seeding and proliferation of hiPSCs. We also observed an altered cell cycle profile and increase in apoptosis in hiPSCs in the absence of insulin. Furthermore, we confirmed phosphorylation of key components of insulin signaling pathway in the presence of insulin and demonstrated the significant effect of insulin on regulation of the mRNA transcriptome of hiPSCs.

conclusionInsulin is a major regulator of seeding, proliferation, phosphorylation and mRNA transcriptome in hiPSCs. Collectively, our work furthers our understanding of human pluripotency and paves the way for future studies that use hiPSCs for modeling genetic ailments affecting insulin signaling pathways.

Indexed as

ApoptosisCell Cycle CheckpointsCell ProliferationHumansInduced Pluripotent Stem CellsInsulinPhosphorylationReceptor, IGF Type 1RNA, MessengerTranscriptomeIGF1R protein, humanInsulinReceptor, IGF Type 1RNA, MessengerApoptosisInsulinInsulin signalingPluripotent stem cellsProliferationTranscriptional regulation

Identifiers

PMID31358052
PMCPMC6664730
OpenAlexW2965567941

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.