ArticlePLoS pathogens2019
In vivo proteomics identifies the competence regulon and AliB oligopeptide transporter as pathogenic factors in pneumococcal meningitis.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 30 citations in OpenAlex.
- Pneumolysin induces platelet destruction, not platelet activation, which can be prevented by immunoglobulin preparations in vitro.Blood advances · 2020Trial
- A synopsis of competence-regulated virulence inVirulence · 2026Review
- Temporal and Spatial Profiling ofProteomes · 2025Article
- Structure, Function, and Regulation of LytA: TheMicroorganisms · 2025Review
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- AliC and AliD of nonencapsulatedFrontiers in cellular and infection microbiology · 2025Article
- Serological Profiling of Pneumococcal Proteins Reveals Unique Patterns of Acquisition, Maintenance, and Waning of Antibodies Throughout Life.The Journal of infectious diseases · 2024Article
- Pneumococcal competence is a populational health sensor driving multilevel heterogeneity in response to antibiotics.Nature communications · 2024Article
- Molecular and structural basis of oligopeptide recognition by the Ami transporter system in pneumococci.PLoS pathogens · 2024Article
- Lipoteichoic Acids Are Essential for Pneumococcal Colonization and Membrane Integrity.Journal of innate immunity · 2024Article
- Article
- Article
- Competence remodels the pneumococcal cell wall exposing key surface virulence factors that mediate increased host adherence.PLoS biology · 2023Article
- Pneumolysin promotes host cell necroptosis and bacterial competence during pneumococcal meningitis as shown by whole-animal dual RNA-seq.Cell reports · 2022Article
- Differences in genetic flux in invasiveHeliyon · 2022Article
- Review
- Hydrogen Peroxide Is Crucial for NLRP3 Inflammasome-Mediated IL-1β Production and Cell Death in Pneumococcal Infections of Bronchial Epithelial Cells.Journal of innate immunity · 2022Article
- The Two-Component System 09 ofMicroorganisms · 2021Article
- The Two-Component System 09 Regulates Pneumococcal Carbohydrate Metabolism and Capsule Expression.Microorganisms · 2021Article
- Diminished Pneumococcal-Specific CD4Frontiers in aging · 2021Article
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus pneumoniae (pneumococci) is a leading cause of severe bacterial meningitis in many countries worldwide. To characterize the repertoire of fitness and virulence factors predominantly expressed during meningitis we performed niche-specific analysis of the in vivo proteome in a mouse meningitis model, in which bacteria are directly inoculated into the cerebrospinal fluid (CSF) cisterna magna. We generated a comprehensive mass spectrometry (MS) spectra library enabling bacterial proteome analysis even in the presence of eukaryotic proteins. We recovered 200,000 pneumococci from CSF obtained from meningitis mice and by MS we identified 685 pneumococci proteins in samples from in vitro filter controls and 249 in CSF isolates. Strikingly, the regulatory two-component system ComDE and substrate-binding protein AliB of the oligopeptide transporter system were exclusively detected in pneumococci recovered from the CSF. In the mouse meningitis model, AliB-, ComDE-, or AliB-ComDE-deficiency resulted in attenuated meningeal inflammation and disease severity when compared to wild-type pneumococci indicating the crucial role of ComDE and AliB in pneumococcal meningitis. In conclusion, we show here mechanisms of pneumococcal adaptation to a defined host compartment by a proteome-based approach. Further, this study provides the basis of a promising strategy for the identification of protein antigens critical for invasive disease caused by pneumococci and other meningeal pathogens.
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