ArticleJournal of proteome research2019
Biochemical Reduction of the Topology of the Diverse WDR76 Protein Interactome.
Article in Journal of proteome research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Two WD40 proteins, AhWD40-170 and AhWD40-171, referred to regulation of anthocyanin accumulation in peanut (Arachis hypogaea L.).BMC plant biology · 2025Article
- An integrated structural model of the DNA damage-responsive H3K4me3 binding WDR76:SPIN1 complex with the nucleosome.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- DIP-MS: ultra-deep interaction proteomics for the deconvolution of protein complexes.Nature methods · 2024Article
- Genome-Wide Identification, Evolution, and Expression Analysis of the WD40 Subfamily inInternational journal of molecular sciences · 2023Article
- Article
- CRISPR screens reveal genetic determinants of PARP inhibitor sensitivity and resistance in prostate cancer.Nature communications · 2023Article
- Genome-Wide Identification of WD40 Proteins inGenes · 2023Article
- Genome-Wide Analysis of Anthocyanin Biosynthesis Regulatory WD40 GeneFrontiers in plant science · 2022Article
- The role of WDR76 protein in human diseases.Bosnian journal of basic medical sciences · 2021Review
- Identification of hot regions in hub protein-protein interactions by clustering and PPRA optimization.BMC medical informatics and decision making · 2021Article
- Identification of stem cells from large cell populations with topological scoring.Molecular omics · 2021Article
- The correlation of WDR76 expression with survival outcomes and immune infiltrates in lung adenocarcinoma.PeerJ · 2021Article
- Genome-wide identification of WD40 genes reveals a functional diversification of COP1-like genes in Rosaceae.Plant molecular biology · 2020Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
A hub protein in protein interaction networks will typically have a large number of diverse interactions. Determining the core interactions and the function of such a hub protein remains a significant challenge in the study of networks. Proteins with WD40 repeats represent a large class of proteins that can be hub proteins. WDR76 is a poorly characterized WD40 repeat protein with possible involvement in DNA damage repair, cell-cycle progression, apoptosis, gene expression regulation, and protein quality control. WDR76 has a large and diverse interaction network that has made its study challenging. Here we rigorously carry out a series of affinity purification coupled to mass spectrometry (AP-MS) analyses to map out the WDR76 interactome through different biochemical conditions. We apply AP-MS analysis coupled to size-exclusion chromatography to resolve WDR76-based protein complexes. Furthermore, we also show that WDR76 interacts with the CCT complex via its WD40 repeat domain and with DNA-PK-KU, PARP1, GAN, SIRT1, and histones outside of the WD40 domain. An evaluation of the stability of WDR76 interactions led to focused and streamlined reciprocal analyses that validate the interactions with GAN and SIRT1. Overall, the approaches used to study WDR76 would be valuable to study other proteins containing WD40 repeat domains, which are conserved in a large number of proteins in many organisms.
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