ArticleStem cell reports2019
Elevated Exogenous Pyruvate Potentiates Mesodermal Differentiation through Metabolic Modulation and AMPK/mTOR Pathway in Human Embryonic Stem Cells.
Article in Stem cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Adjusting PSC culture for neural organoid generation.Stem cell reports · 2026Article
- Biomimetic ECM collagen hydrogel induces chondrogenic differentiation of BMSCs by activating autophagy through POU5F1-mediated AMPK/mTOR signaling pathway.Theranostics · 2026Article
- Metabolic regulation in the senescence process of stem cells.Stem cells translational medicine · 2025Review
- Signaling Pathways in Human Blastocyst Development: From Molecular Mechanisms to In Vitro Optimization.Journal of developmental biology · 2025Review
- Stereotyped Subclones Revealed by High-Density Single-Cell Lineage Tracing Support Robust Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Serine synthesis pathway regulates cardiac differentiation from human pluripotent stem cells.iScience · 2025Article
- Dynamic Interplay Between Autophagy and Oxidative Stress in Stem Cells: Implications for Regenerative Medicine.Antioxidants (Basel, Switzerland) · 2025Review
- Metabolic patterns predispose human pluripotent stem cells to spatial organization of cell fate.Frontiers in cell and developmental biology · 2025Article
- The modulation of calcium and chloride channels induces cardiomyocytes from human pluripotent stem cells.International journal of biological sciences · 2025Article
- Review
- ERK5 promotes autocrine expression to sustain mitogenic balance for cell fate specification in human pluripotent stem cells.Stem cell reports · 2024Article
- Proteomic analysis of meropenem-induced outer membrane vesicles released by carbapenem-resistantMicrobiology spectrum · 2024Article
- Molecular mechanisms of cellular metabolic homeostasis in stem cells.International journal of oral science · 2023Review
- mTOR signalling pathway in stem cell bioactivities and angiogenesis potential.Cell proliferation · 2023Review
- Early Differentiation Signatures in Human Induced Pluripotent Stem Cells Determined by Non-Targeted Metabolomics Analysis.Metabolites · 2023Article
- BMP4 aggravates mitochondrial dysfunction of HRMECs.Heliyon · 2023Article
- Metabolomic characterization of congenital microtia: a possible analysis for early diagnosis.Annals of translational medicine · 2022Article
- Article
- Dietary calcium pyruvate could improve growth performance and reduce excessive lipid deposition in juvenile golden pompano (Trachinotus ovatus) fed a high fat diet.Fish physiology and biochemistry · 2022Article
- Thyroid hormone enhances stem cell maintenance and promotes lineage-specific differentiation in human embryonic stem cells.Stem cell research & therapy · 2022Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyruvate is a key metabolite in glycolysis and the tricarboxylic acid (TCA) cycle. Exogenous pyruvate modulates metabolism, provides cellular protection, and is essential for the maintenance of human preimplantation embryos and human embryonic stem cells (hESCs). However, little is known about how pyruvate contributes to cell-fate determination during epiblast stage. In this study, we used hESCs as a model to demonstrate that elevated exogenous pyruvate shifts metabolic balance toward oxidative phosphorylation in both maintenance and differentiation conditions. During differentiation, pyruvate potentiates mesoderm and endoderm lineage specification. Pyruvate production and its mitochondrial metabolism are required in BMP4-induced mesoderm differentiation. However, the TCA-cycle metabolites do not have the same effect as pyruvate on differentiation. Further study shows that pyruvate increases AMP/ATP ratio, activates AMPK, and modulates the mTOR pathway to enhance mesoderm differentiation. This study reveals that exogenous pyruvate not only controls metabolism but also modulates signaling pathways in hESC differentiation.
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