ArticleRedox biology2019
Malondialdehyde interferes with the formation and detection of primary carbonyls in oxidized proteins.
Article in Redox biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 79 citations in OpenAlex.
- Article
- Rapeseed Protein Isolate as a Sustainable Alternative to Soy Protein: A Case Study on Chicken Pâtés.Foods (Basel, Switzerland) · 2025Article
- The Role of Natural Low Molecular Weight Dicarbonyls in Atherogenesis and Diabetogenesis.Reviews in cardiovascular medicine · 2024Review
- Changes in Novel Biomarkers for Protein Oxidation in Pork Patties under Different Cooking Methods.Foods (Basel, Switzerland) · 2024Article
- Lipid-Induced Oxidative Modifications Decrease the Bioactivities of Collagen Hydrolysates from Fish Skin: The Underlying Mechanism Based on the Proteomic Strategy.Foods (Basel, Switzerland) · 2024Article
- Nisin lantibiotic prevents NAFLD liver steatosis and mitochondrial oxidative stress following periodontal disease by abrogating oral, gut and liver dysbiosis.NPJ biofilms and microbiomes · 2024Article
- In Silico and In Vivo Evaluation of the Maqui Berry (Metabolites · 2023Article
- Malondialdehyde-Induced Post-Translational Modification of Human Hemoglobin.Journal of proteome research · 2023Article
- Insight into the Interaction of Malondialdehyde with Rabbit Meat Myofibrillar Protein: Fluorescence Quenching and Protein Oxidation.Foods (Basel, Switzerland) · 2023Article
- Malondialdehyde as an Important Key Factor of Molecular Mechanisms of Vascular Wall Damage under Heart Diseases Development.International journal of molecular sciences · 2022Review
- Dicarbonyl-Dependent Modification of LDL as a Key Factor of Endothelial Dysfunction and Atherosclerotic Vascular Wall Damage.Antioxidants (Basel, Switzerland) · 2022Review
- Protein carbonylation in food and nutrition: a concise update.Amino acids · 2022Article
- Molecular mechanisms of the disturbance caused by malondialdehyde on probiotic Lactobacillus reuteri PL503.Microbial biotechnology · 2022Article
- Effectiveness of Sprayed Bioactive Fruit Extracts in Counteracting Protein Oxidation in Lamb Cutlets Subjected to a High-Oxygen MAP.Foods (Basel, Switzerland) · 2020Article
- The Advanced Lipoxidation End-Product Malondialdehyde-Lysine in Aging and Longevity.Antioxidants (Basel, Switzerland) · 2020Review
- A Chemometric Approach to Establish Underlying Connections between Lipid and Protein Oxidation and Instrumental Color and Texture Characteristics in Brazilian Dry-cured Loin.Foods (Basel, Switzerland) · 2020Article
- The Skin Epilipidome in Stress, Aging, and Inflammation.Frontiers in endocrinology · 2020Review
- Benefits of Magnesium Supplementation to Broiler Subjected to Dietary and Heat Stress: Improved Redox Status, Breast Quality and Decreased Myopathy Incidence.Antioxidants (Basel, Switzerland) · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonylation is one of the most remarkable expressions of the oxidative damage to proteins and the DNPH method the most common procedure to assess protein oxidation in biological samples. The present study was elicited by two hypotheses: i) is malondialdehyde, as a reactive dicarbonyl, able to induce the formation of allysine through a Maillard-type reaction? and ii) to which extent does the attachment of MDA to proteins interfere in the assessment of protein carbonyls using the DNPH method? Human serum albumin (HSA), human hemoglobin (HEM) and β-lactoglobulin (LAC) (5 mg/mL) were incubated with MDA (0.25 mM) for 24 h at 37 °C (HSA and HEM) or 80 °C (LAC). Results showed that MDA was unable to induce oxidative deamination of lysine residues and instead, formed stable and fluorescent adducts with proteins. Such adducts were tagged by the DNPH method, accounting for most of the protein hydrazones quantified. This interfering effect was observed in a wide range of MDA concentrations (0.05-1 mM). Being aware of its limitations, protein scientists should accurately interpret results from the DNPH method, and apply, when required, other methodologies such as chromatographic methods to detect specific primary oxidation products such as allysine.
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Registered trials
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