ArticleThe Plant journal : for cell and molecular biology2019
Multi-targeting of viral RNAs with synthetic trans-acting small interfering RNAs enhances plant antiviral resistance.
Article in The Plant journal : for cell and molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Envisaging the role of plant Small RNAs: A comprehensive review on antiviral defense mechanisms.Molecular biology reports · 2026Review
- Precision RNAi in Tomato Using Synthetic Trans-Acting Small Interfering RNAs Derived From Minimal Precursors.Plant biotechnology journal · 2026Article
- MicroRNA-Induced Gene Silencing (MIGS): A Tool for Multi-Gene Silencing and Targeting Viruses in Plants.Plant biotechnology journal · 2026Article
- Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing.The Plant journal : for cell and molecular biology · 2026Article
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Syn-tasiR-VIGS: virus-based targeted RNAi in plants by synthetic trans-acting small interfering RNAs derived from minimal precursors.Nucleic acids research · 2025Article
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- Artificial miRNAs and target-mimics as potential tools for crop improvement.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- syn-tasiRnas targeting the coat protein of potato virus Y confer antiviral resistance inPlant signaling & behavior · 2024Article
- Transgene-free, virus-based gene silencing in plants by artificial microRNAs derived from minimal precursors.Nucleic acids research · 2023Article
- Advances and Prospects of Virus-Resistant Breeding in Tomatoes.International journal of molecular sciences · 2023Review
- Large Artificial microRNA Cluster Genes Confer Effective Resistance against Multiple Tomato Yellow Leaf Curl Viruses in Transgenic Tomato.Plants (Basel, Switzerland) · 2023Article
- SilencingPotato research · 2023Article
- Targeting of genomic and negative-sense strands of viral RNA contributes to antiviral resistance mediated by artificial miRNAs and promotes the emergence of complex viral populations.Molecular plant pathology · 2022Article
- Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions.Stress biology · 2022Review
- Exosomal mediated signal transduction through artificial microRNA (amiRNA): A potential target for inhibition of SARS-CoV-2.Cellular signalling · 2022Review
- Regulation of small RNA-mediated high temperature stress responses in crop plants.Plant cell reports · 2022Review
- Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber.International journal of molecular sciences · 2021Article
- Roles of small RNAs in crop disease resistance.Stress biology · 2021Review
- RNA-Based Technologies for Engineering Plant Virus Resistance.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi)-based tools are used in multiple organisms to induce antiviral resistance through the sequence-specific degradation of target RNAs by complementary small RNAs. In plants, highly specific antiviral RNAi-based tools include artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs). syn-tasiRNAs have emerged as a promising antiviral tool allowing for the multi-targeting of viral RNAs through the simultaneous expression of several syn-tasiRNAs from a single precursor. Here, we compared in tomato plants the effects of an amiRNA construct expressing a single amiRNA and a syn-tasiRNA construct expressing four different syn-tasiRNAs against Tomato spotted wilt virus (TSWV), an economically important pathogen affecting tomato crops worldwide. Most of the syn-tasiRNA lines were resistant to TSWV, whereas the majority of the amiRNA lines were susceptible and accumulated viral progenies with mutations in the amiRNA target site. Only the two amiRNA lines with higher amiRNA accumulation were resistant, whereas resistance in syn-tasiRNA lines was not exclusive of lines with high syn-tasiRNA accumulation. Collectively, these results suggest that syn-tasiRNAs induce enhanced antiviral resistance because of the combined silencing effect of each individual syn-tasiRNA, which minimizes the possibility that the virus simultaneously mutates all different target sites to fully escape each syn-tasiRNA.
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