ArticleGenes & genomics2019
Comparison of library construction kits for mRNA sequencing in the Illumina platform.
Article in Genes & genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Decoding the amniotic membrane transcriptome during equine ascending placentitis.Scientific reports · 2025Article
- Differentially expressed platelet activation-related genes in dogs with stage B2 myxomatous mitral valve disease.BMC veterinary research · 2023Article
- Screening for diagnostic targets in tuberculosis and study on its pathogenic mechanism based on mRNA sequencing technology and miRNA-mRNA-pathway regulatory network.Frontiers in immunology · 2023Article
- Whole-Transcriptome Profiling on Small FFPE Samples: Which Sequencing Kit Should Be Used?Current issues in molecular biology · 2022Article
- Targeting DNAJC19 overcomes tumor growth and lung metastasis in NSCLC by regulating PI3K/AKT signaling.Cancer cell international · 2021Article
- Small Noncoding RNA Signatures for Determining the Developmental Potential of an Embryo at the Morula Stage.International journal of molecular sciences · 2020Article
- Transcriptome Analysis Reveals the Effects of Troxerutin and Cerebroprotein Hydrolysate Injection on Injured Spinal Cords in Rats.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Comparison of Two Illumina Whole Transcriptome RNA Sequencing Library Preparation Methods Using Human Cancer FFPE Specimens.Technology in cancer research & treatmentArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe emergence of next-generation sequencing (NGS) technologies has made a tremendous contribution to the deciphering and significance of transcriptome analysis in biological fields. Since the advent of NGS technology in 2007, Illumina, Inc. has provided one of the most widely used sequencing platforms for NGS analysis.
objectiveAlthough reagents and protocols provided by Illumina are adequately performed in transcriptome sequencing, recently, alternative reagents and protocols which are relatively cost effective are accessible. However, the kits derived from various manufacturers have advantages and disadvantages when researchers carry out the transcriptome library construction.
methodsWe compared them using a variety of protocols to produce Illumina-compatible libraries based on transcriptome. Three different mRNA sequencing kits were selected for this study: TruSeq
resultsThe quality and quantity of sequencing data obtained through the NGS technique were strongly influenced by the type of the sequencing library kits. It suggests that for transcriptome studies, researchers should select a suitable library construction kit according to the goal and resources of experiments.
conclusionThe present work will help researchers to choose the right sequencing library construction kit for transcriptome analyses.
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