ArticleScientific reports2019
Scalable Labeling for Cytoarchitectonic Characterization of Large Optically Cleared Human Neocortex Samples.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Optical Tissue Clearing and Small-Molecule Labeling of Paraffin-Embedded Breast Cancer and Axillary Lymph Node Human Tissue Samples.Bio-protocol · 2026Article
- aDISCO: A clearing method to enable 3D microscopy of large archival paraffin-embedded human tissue blocks.Science advances · 2026Article
- From identity to function: unveiling the cellular complexity of hypothalamic feeding circuits.Reviews in endocrine & metabolic disorders · 2026Review
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- An open-source photobleacher for fluorescence imaging of large pigment-rich tissues.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- ht-MASH: a high-throughput, cost-effective, and robust protocol for microscopic 3D imaging of human angio- and cytoarchitecture in large human brain samples.Anatomical science international · 2025Article
- Advances in tissue optical clearing for 3D imaging in large animal.Frontiers of optoelectronics · 2025Review
- Delivery of a fibrin-binding hemostatic polymer ameliorates neurovascular damage and neural tissue loss after traumatic brain injury.Science advances · 2025Article
- Unlocking the potential of large-scale 3D imaging with tissue clearing techniques.Microscopy (Oxford, England) · 2025Review
- Current and future applications of light-sheet imaging for identifying molecular and developmental processes in autism spectrum disorders.Molecular psychiatry · 2024Review
- Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for cleared samples.Nature communications · 2024Article
- The Blood-Brain Barrier in Both Humans and Rats: A Perspective From 3D Imaging.International journal of biomedical imaging · 2024Article
- Reflective multi-immersion microscope objectives inspired by the Schmidt telescope.Nature biotechnology · 2024Article
- SHARD: an improved method for staining and visualizing multiplex immunofluorescence in optically cleared postmortem human brain tissue.Frontiers in neuroscience · 2024Article
- The Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samples.bioRxiv : the preprint server for biology · 2023Article
- On-line clearing and staining method for the efficient optical imaging of large volume samples at the cellular resolution.Biomedical optics express · 2023Article
- A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy.International journal of molecular sciences · 2023Review
- Efficient 3D light-sheet imaging of very large-scale optically cleared human brain and prostate tissue samples.Communications biology · 2023Article
- Detection and Morphological Analysis of Micro-Ruptured Cortical Arteries in Subdural Hematoma: Three-Dimensional Visualization Using the Tissue-Clearing Clear, Unobstructed, Brain/Body Imaging Cocktails and Computational Analysis Method.Diagnostics (Basel, Switzerland) · 2022Article
- Scalable tissue labeling and clearing of intact human organs.Nature protocols · 2022Review
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and labeling human brain tissue through all cortical layers and significant portions of a cortical area, has so far remained extremely challenging, especially for formalin fixed adult cortical tissue. Here, we present MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling approach for optically cleared human cortex samples, which can be applied to large (up to 5 mm thick) formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dye protocols in combination with new refractive index matching solutions allows deep volume imaging. This greatly reduces time and cost of imaging cytoarchitecture in thick samples and enables classification of cytoarchitectonic layers over the full cortical depth. We demonstrate application of MASH to large archival samples of human visual areas, characterizing cortical architecture in 3D from the scale of cortical areas to that of single cells. In combination with scalable light sheet imaging and data analysis, MASH could open the door to investigation of large human cortical systems at cellular resolution and in the context of their complex 3-dimensional geometry.
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