ArticleScientific reports2019
3D-Imaging of Whole Neuronal and Vascular Networks of the Human Dental Pulp via CLARITY and Light Sheet Microscopy.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 67 citations in OpenAlex.
- Strategies to Microengineer Vascularized, Mineralized, and Immunomodulatory Niches for Dental Regeneration.Journal of endodontics · 2026Article
- Dental Pulp Stem Cells for the Management of Plaque Biofilm-Associated Infections : A Review.International dental journal · 2026Review
- Effect of Intracapsular Pressure on Pulp Sensitivity in Teeth Affected by Jaw Cysts: A Clinical Study Combined With Finite Element Analysis.International dental journal · 2026Article
- Human Atlas of Tooth Decay Progression: Identification of Cellular Mechanisms Driving the Switch from Dental Pulp Repair Toward Irreversible Pulpitis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- CGRP-mediated neuro-vascular-pulp cell crosstalk is essential for dental pulp repair.Frontiers in cell and developmental biology · 2026Article
- Histomorphological and ultrastructural analysis of vibrissal follicle-sinus complexes in the dog (Canis lupus familiaris).PloS one · 2026Article
- EZH2-Mediated Epigenetic Modifications Induce Pyroptosis in Dental Pulp Endothelial Cells via Activation of NLRP6 Inflammasome During Pulpitis Development.Mediators of inflammation · 2026Article
- Tooth Pulp Afferents and Transient Receptor Potential (TRP) Ion Channels as Key Regulators of Pulp Homeostasis, Inflammation, and Pain.International journal of molecular sciences · 2025Review
- EZH2 knockout in mice activates STAT3 signalling via STAT3 methylation and modulates ferroptosis in pulpitis-affected dental pulp vascular endothelial cells: A laboratory investigation.International endodontic journal · 2025Article
- The Influence of the Periodontal Breakdown over the Amount of Orthodontic Force Reaching the Dental Pulp and NVB During Orthodontic Movements-A Biomechanical Finite Element Analysis.Journal of clinical medicine · 2025Article
- A retrospective, cross-sectional analysis of the dental status and needs of patients taking vascular endothelial growth factor (VEGF) antagonists.Clinical oral investigations · 2025Article
- The tooth transformer® revolution: autologous dentin biomaterials and platelet concentrates in oral regeneration. A parallel narrative systematic review.Frontiers in dental medicine · 2025Review
- Five Numerical Methods to Assess the Ischemic Risks in Dental Pulp and Neuro-Vascular Bundle Under Orthodontic Movements in Intact Periodontium In Vitro.Dentistry journal · 2024Article
- The Amount of Orthodontic Force Reaching the Dental Pulp and Neuro-Vascular Bundle During Orthodontic Movements in the Intact Periodontium.Medicina (Kaunas, Lithuania) · 2024Article
- Periodontal Breakdown, Orthodontic Movements and Pulpal Ischemia Correlations-A Comparison Between Five Study Methods.Journal of clinical medicine · 2024Article
- Ischemic Risks Induced by Larger Orthodontic Forces on Dental Pulp and Neuro-Vascular Bundle in Reduced Periodontium.Journal of clinical medicine · 2024Article
- The Effect of Larger Orthodontic Forces and Movement Types over a Dental Pulp and Neuro-Vascular Bundle of Lower Premolars in Intact Periodontium-A Numerical Analysis.Dentistry journal · 2024Article
- Sensory nerves drive migration of dental pulp stem cells via the CGRP-Ramp1 axis in pulp repair.Cellular and molecular life sciences : CMLS · 2024Article
- Mechanobiology of Dental Pulp Cells.Cells · 2024Review
- The Role of Sensory Nerves in Dental Pulp Homeostasis: Histological Changes and Cellular Consequences after Sensory Denervation.International journal of molecular sciences · 2024Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Direct visualization of the spatial relationships of the dental pulp tissue at the whole-organ has remained challenging. CLARITY (Clear Lipid-exchanged Acrylamide Tissue hYdrogel) is a tissue clearing method that has enabled successful 3-dimensional (3D) imaging of intact tissues with high-resolution and preserved anatomic structures. We used CLARITY to study the whole human dental pulp with emphasis on the neurovascular components. Dental pulps from sound teeth were CLARITY-cleared, immunostained for PGP9.5 and CD31, as markers for peripheral neurons and blood vessels, respectively, and imaged with light sheet microscopy. Visualization of the whole dental pulp innervation and vasculature was achieved. Innervation comprised 40% of the dental pulp volume and the vasculature another 40%. Marked innervation morphological differences between uni- and multiradicular teeth were found, also distinct neurovascular interplays. Quantification of the neural and vascular structures distribution, diameter and area showed that blood vessels in the capillary size range was twice as high as that of nerve fibers. In conclusion whole CLARITY-cleared dental pulp samples revealed 3D-morphological neurovascular interactions that could not be visualized with standard microscopy. This represents an outstanding tool to study the molecular and structural intricacies of whole dental tissues in the context of disease and treatment methods.
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