ArticleMicromachines2019
Paper-Based Resazurin Assay of Inhibitor-Treated Porcine Sperm.
Article in Micromachines, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Optimization of Black Boar Sperm Cryopreservation Efficiency with Antioxidant-Rich Plant Extracts from Djulis (Animals : an open access journal from MDPI · 2025Article
- A Mobile Sperm Analyzer with User-Friendly Microfluidic Chips for Rapid On-Farm Semen Evaluation.Biosensors · 2025Article
- Theoretical Evaluation of Fluorinated Resazurin Derivatives for In Vivo Applications.Molecules (Basel, Switzerland) · 2024Article
- Maternal restricted- and over- feeding during gestation perturb offspring sperm epigenome in sheep.Reproduction (Cambridge, England) · 2023Article
- Detection of Microorganisms in Body Fluids via MTT-PMS Assay.Diagnostics (Basel, Switzerland) · 2021Article
- Visible colorimetric growth indicators of Neisseria gonorrhoeae for low-cost diagnostic applications.PloS one · 2021Article
- Portable Device for Quick Detection of Viable Bacteria in Water.Micromachines · 2020Article
- Editorial for the Special Issue on Point-of-Care Devices.Micromachines · 2020Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Porcine sperm motility was assessed via resazurin reduction color change in sperm cells using a novel paper-based assay of our own design. We applied mixtures of resazurin solution and porcine semen onto hydrophilic test circles on our paper-based device and investigated the resulting reduction reaction expressed as red and blue color intensity (RBCI). We quantified this reaction using a blue/pink color ratio from our 8 × 3 = 24 bit RGB color image. To examine enzymatic reactivity in sperm cells, we used two inhibitors: 3-Nitropropanoic acid (3-NPA) and 3-Bromopyruvic acid (3-BP). 3-NPA inhibits the citric acid cycle and electron transfer reaction in mitochondria, but did not strongly reduce sperm motility in our tests. 3-BP decreases reactivity of both mitochondrial electron transfer and glycolytic enzymes in cytosol, which significantly lowers porcine sperm motility. RBCIs of 3-NPA- and 3-BP-treated samples were significantly lower compared to our untreated control (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.