ReviewMetabolites2019
Pre-Analytical Factors that Affect Metabolite Stability in Human Urine, Plasma, and Serum: A Review.
Review in Metabolites, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 98 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
98 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- A priori sample size determination and power analysis in metabolic phenotyping and integrative metabolomics: an application framework based on a systematic review of literature.Metabolomics : Official journal of the Metabolomic Society · 2026Pooled it
- Metabolomic insights into late-life depression: a systematic review.BMC geriatrics · 2025Pooled it
- Metabolomics in Primary Open Angle Glaucoma: A Systematic Review and Meta-Analysis.Frontiers in neuroscience · 2022Pooled it
- Plasma Metabolites Link Dietary Patterns to Stroke Risk.Annals of neurology · 2023Trial
- Determinants of the plasma metabolome: cross-sectional and longitudinal associations over six years in the NESDA cohort.EBioMedicine · 2026Article
- Elevated urinary succinate in women with symptom-defined overactive bladder without clinically recognized cardiometabolic disease: a cross-sectional study.BMC women's health · 2026Article
- Systematic evaluation of pre-analytical variables on synovial fluid metabolomic profiles using GC-ToF-MS and UHPLC-MS.The Analyst · 2026Article
- Deciphering the Arterial and Venous Blood Bacterial DNA Profile: Pioneering Insights into Coronary Heart Disease Etiology and Progression.Microorganisms · 2026Article
- Advances in biomarkers for diagnosing and prognosticating disorders of consciousness.Frontiers in neuroscience · 2026Review
- Plant-based dietary biomarkers: from discovery to validation using mass spectrometry-based metabolomics.Frontiers in nutrition · 2026Review
- Metabolomics biomarkers for precision psychiatry.Frontiers in psychiatry · 2026Review
- Mass Spectrometry as a Tool for Metabolomics in Cancer.Advances in experimental medicine and biology · 2026Review
- Integrated genomics and metabolomics to identify cause-specific biomarkers for chronic kidney disease in a Korean population.Kidney research and clinical practice · 2026Article
- Disease and Medication Context Shape Ex Vivo Metabolite Stability: A Pilot Study in Systemic Lupus Erythematosus.Metabolites · 2025Article
- Metabolomic Alterations in Patients with Obesity and the Impact of Metabolic Bariatric Surgery: Insights for Future Research.Metabolites · 2025Review
- Reproducibility of Plasma Metabolome over 1 Year in a Population-Based Cohort of Black Breast Cancer Survivors.Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology · 2025Article
- A pilot study on hemodynamically stable isolated chest trauma patients reveals dysregulation of oxidative metabolism.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Comparing 17β-estradiol and progesterone concentrations in young, physically active females: Insights from plasma versus serum analysis.Experimental physiology · 2025Article
- Strategies for discovering novel hepatocellular carcinoma biomarkers.World journal of hepatology · 2025Review
- Evaluation of solutions for stabilizing feces in metabolomics studies using GC × GC-TOFMS.Metabolomics : Official journal of the Metabolomic Society · 2025Article
38 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metabolomics provides a comprehensive assessment of numerous small molecules in biological samples. As it integrates the effects of exogenous exposures, endogenous metabolism, and genetic variation, metabolomics is well-suited for studies examining metabolic profiles associated with a variety of chronic diseases. In this review, we summarize the studies that have characterized the effects of various pre-analytical factors on both targeted and untargeted metabolomic studies involving human plasma, serum, and urine and were published through 14 January 2019. A standardized protocol was used for extracting data from full-text articles identified by searching PubMed and EMBASE. For plasma and serum samples, metabolomic profiles were affected by fasting status, hemolysis, collection time, processing delays, particularly at room temperature, and repeated freeze/thaw cycles. For urine samples, collection time and fasting, centrifugation conditions, filtration and the use of additives, normalization procedures and multiple freeze/thaw cycles were found to alter metabolomic findings. Consideration of the effects of pre-analytical factors is a particularly important issue for epidemiological studies where samples are often collected in nonclinical settings and various locations and are subjected to time and temperature delays prior being to processed and frozen for storage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.