ArticleMetabolic engineering2019
Developing a pyruvate-driven metabolic scenario for growth-coupled microbial production.
Article in Metabolic engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Development and Protective Evaluation of an O18-CRM197 Glycoconjugate Candidate Preparation Against APEC O18 Challenge in SPF Chickens.Microorganisms · 2026Article
- A non‑natural shortcut biosynthetic route enables de novo microbial production of 3‑hydroxyanthranilic and quinolinic acids.World journal of microbiology & biotechnology · 2026Article
- Optimizing L-Tryptophan Production inJournal of microbiology and biotechnology · 2025Article
- Balancing Cell Growth and Product Synthesis for Efficient Microbial Cell Factories.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Engineering Escherichia coli for Efficient Production of L-Tryptophan.Applied biochemistry and biotechnology · 2025Article
- Harnessing microbial heterogeneity for improved biosynthesis fueled by synthetic biology.Synthetic and systems biotechnology · 2025Review
- A growth-coupling strategy for improving the stability of terpenoid bioproduction in Escherichia coli.Microbial cell factories · 2024Article
- Novel cathodic and anodic dual-emitting electrochemiluminescence of Ru(bpy)Mikrochimica acta · 2024Article
- Concentration Recognition-Based Auto-Dynamic Regulation System (CRUISE) Enabling Efficient Production of Higher Alcohols.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Characterization of anthranilic acid produced by Virgibacillus salarius MML1918 and its bio-imaging application.World journal of microbiology & biotechnology · 2024Article
- A self-regulated network for dynamically balancing multiple precursors in complex biosynthetic pathways.Metabolic engineering · 2024Article
- Dynamic Metabolic Control: From the Perspective of Regulation Logic.Synthetic biology and engineering · 2023Article
- Dynamic control of the distribution of carbon flux between cell growth and butyrate biosynthesis in Escherichia coli.Applied microbiology and biotechnology · 2021Article
- Overproduction of D-pantothenic acid via fermentation conditions optimization and isoleucine feeding from recombinant3 Biotech · 2021Article
- Novel Mode Engineering for β-Alanine Production inMicroorganisms · 2021Article
- Engineering of Saccharomyces cerevisiae for anthranilate and methyl anthranilate production.Microbial cell factories · 2021Article
- Optimization of aACS synthetic biology · 2021Article
- Control strategies to manage trade-offs during microbial production.Current opinion in biotechnology · 2020Review
- Recent advances in improving metabolic robustness of microbial cell factories.Current opinion in biotechnology · 2020Review
- The future of self-selecting and stable fermentations.Journal of industrial microbiology & biotechnology · 2020Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Microbial-based chemical synthesis serves as a promising approach for sustainable production of industrially important products. However, limited production performance caused by metabolic burden or genetic variations poses one of the major challenges in achieving an economically viable biomanufacturing process. To address this issue, one superior strategy is to couple the product synthesis with cellular growth, which renders production obligatory for cell survival. Here we create a pyruvate-driven metabolic scenario in engineered Escherichia coli for growth-coupled bioproduction, with which we demonstrate its application in boosting production of anthranilate and its derivatives. Deletion of a minimal set of endogenous pyruvate-releasing pathways engenders anthranilate synthesis as the salvage route for pyruvate generation to support cell growth, concomitant with simultaneous anthranilate production. Further introduction of native and non-native downstream pathways affords production enhancement of two anthranilate-derived high-value products including L-tryptophan and cis, cis-muconic acid from different carbon sources. The work reported here presents a new growth-coupled strategy with demonstrated feasibility for promoting microbial production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.