Evidence map›Paper›PMID 31346317›Full record

ArticleCancer cell international2019

Circadian protein BMAL1 promotes breast cancer cell invasion and metastasis by up-regulating matrix metalloproteinase9 expression.

Jian Wang, Shujing Li, Xiahui Li, Bowen Li, Yanan Li, Kangkai Xia, Yuxi Yang, Sattout Aman, Miao Wang, Huijian Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.

0numbers the graph read from it
0cells of the map it votes in
61citing papers in PubMed
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

61 citing papers in PubMed, 90 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Circadian rhythms and lung cancer biology and immunotherapy: Emerging opportunities and challenges.Chinese medical journal pulmonary and critical care medicine · 2026
    Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Circadian clock and cancer.Military Medical Research · 2026
    Review
  10. Review
  11. Review
  12. Article
  13. Article
  14. Article
  15. Melatonin and Breast Cancer: A Review Article.Chonnam medical journal · 2025
    Review
  16. Article
  17. Review
  18. Review
  19. Review
  20. Article

1 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Jian Wang *Province Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Shujing Li *Province Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Xiahui LiProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Bowen LiProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Yanan LiProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Kangkai XiaProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Yuxi YangProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Sattout AmanProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Miao WangProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Huijian WuProvince Key Laboratory of Protein Modification and Occurrence of Disease, Dalian University of Technology, 2 Ling Gong Road, Dalian, 116024 Liaoning Province China.
Dalian University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMetastasis is an important factor in the poor prognosis of breast cancer. As an important core clock protein, brain and muscle arnt-like 1 (BMAL1) is closely related to tumorigenesis. However, the molecular mechanisms that mediate the role of BMAL1 in invasion and metastasis remain largely unknown. In this study, we investigated the BMAL1 may take a crucial effect in the progression of breast cancer cells.

methodsBMAL1 and MMP9 expression was measured in breast cell lines. Transwell and scratch wound-healing assays were used to detect the movement of cells and MTT assays and clonal formation assays were used to assess cells' proliferation. The effects of BMAL1 on the MMP9/NF-κB pathway were examined by western blotting, co-immunoprecipitation and mammalian two-hybrid.

resultsIn our study, it showed that cell migration and invasion were significantly enhanced when overexpressed BMAL1. Functionally, overexpression BMAL1 significantly increased the mRNA and protein level of matrix metalloproteinase9 (MMP9) and improved the activity of MMP9. Moreover, BMAL1 activated the NF-κB signaling pathway by increasing the phosphorylation of IκB and promoted human MMP9 promoter activity by interacting with NF-kB p65, leading to increased expression of MMP9. When overexpressed BMAL1, CBP (CREB binding protein) was recruited to enhance the activity of p65 and further activate the NF-κB signaling pathway to regulate the expression of its downstream target genes, including MMP9, TNFα, uPA and IL8, and then promote the invasion and metastasis of breast cancer cells.

conclusionsThis study confirmed a new mechanism by which BMAL1 up-regulated MMP9 expression to increase breast cancer metastasis, to provide research support for the prevention and treatment of breast cancer.

Indexed as

BMAL1InvasionMetastasisMMP9NF-κB

Identifiers

PMID31346317
PMCPMC6636133
OpenAlexW2957967073

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.