Evidence map›Paper›PMID 31344405›Full record

ArticleMethods (San Diego, Calif.)2019

Fluorescent probes with high pKa values based on traditional, near-infrared rhodamine, and hemicyanine fluorophores for sensitive detection of lysosomal pH variations.

Wafa Mazi, Rashmi Adhikari, Yibin Zhang, Shuai Xia, Mingxi Fang, Rudy L Luck, Momoko Tajiri, Ashutosh Tiwari, Marina Tanasova, Haiying Liu

Open access · greenAbstract read
In one paragraph

Article in Methods (San Diego, Calif.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Wafa MaziDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States.
Rashmi AdhikariDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States.
Yibin ZhangDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States.
Shuai XiaDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States.
Mingxi FangDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States.
Rudy L LuckDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: rluck@mtu.edu.
Momoko TajiriDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: mtajiri@mtu.edu.
Ashutosh TiwariDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: tiwari@mtu.edu.
Marina TanasovaDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: mtanasov@mtu.edu.
Haiying LiuDepartment of Chemistry, Michigan Technological University, Houghton, MI 49931, United States. Electronic address: hyliu@mtu.edu.
Michigan Technological University · US

Funding

Ratiometric Near-infrared Fluorescent Probes for Lysosomal pH in Living CellsR15GM114751 · NIGMS · MICHIGAN TECHNOLOGICAL UNIVERSITY · PI LIU, HAIYING · 2015 to 2019
$798k
NIGMS NIH HHS R15 GM114751
6 · The paper itself

Abstract

Sterically hindered fluorescent probes (A-C) have been developed by introducing 2-aminophenylboronic acid pinacol ester to a traditional, A, a near-infrared rhodamine dye, B, and a near-infrared hemicyanine dye, C, forming closed spirolactam ring structures. Probe A was non-fluorescent under basic pH conditions whereas probes B and C were moderately fluorescent with fluorescence quantum yields of 9% and 5% in pH 7.4 PBS buffer containing 1% ethanol, respectively. With all probes increasing acidity leads to significant increases in fluorescence at 580 nm, 644 and 744 nm for probes A, B and C with fluorescence quantum yields of 26%, 21% and 10% in pH 4.5 PBS buffer containing 1% ethanol, respectively. Probes A, B and C were calculated to have pK

Indexed as

Spectroscopy, Near-InfraredBoronic AcidsCarbocyaninesCell Line, TumorEstersFluorescenceFluorescent DyesHeLa CellsHumansHuman Umbilical Vein Endothelial CellsHydrogen-Ion ConcentrationLysosomesMolecular ProbesRhodaminesSpironolactoneXanthenes3-aminobenzeneboronic acidBoronic AcidsCarbocyaninesEstersFluorescent DyesMolecular ProbesRhodaminesSpironolactonetetramethylene hemicyanineXanthenesFluorescence imagingFluorescent probesLysosomal pHNear-infrared fluorescenceRhodamine dyes

Identifiers

PMID31344405
PMCPMC6851477
OpenAlexW2963528494

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.