ReviewMethods (San Diego, Calif.)2020
Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens.
Review in Methods (San Diego, Calif.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Cell swelling and upright mounting-based imaging for high-resolution visualization of intracellular trafficking across the BBB using conventional confocal microscopy.Drug delivery · 2026Article
- Wavelength-tunable depletion expands fluorophore compatibility in two-photon STED nanoscopy.Biomedical optics express · 2026Article
- Live-cell STED microscopy enables 50 nm resolution imaging with preserved cell proliferation.Scientific reports · 2026Article
- A comparison of super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium.Journal of microscopy · 2026Article
- Integrating the Contrasting Perspectives Between the Constrained Disorder Principle and Deterministic Optical Nanoscopy: Enhancing Information Extraction from Imaging of Complex Systems.Bioengineering (Basel, Switzerland) · 2026Article
- Self-Driving Microscopes: AI Meets Super-Resolution Microscopy.Small methods · 2026Review
- STED Super-Resolution Microscopy for Studying Actin Cytoskeleton Organization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advancements in Single-Molecule Fluorescence Detection Techniques and Their Expansive Applications in Drug Discovery and Neuroscience.Biosensors · 2025Review
- Super-resolution expansion microscopy in plant roots.The Plant cell · 2025Article
- Spatial organization of translation and translational repression in two phases of germ granules.Nature communications · 2024Article
- Imaging brain tissue architecture across millimeter to nanometer scales.Nature biotechnology · 2024Article
- Nanoscale insights into hematology: super-resolved imaging on blood cell structure, function, and pathology.Journal of nanobiotechnology · 2024Review
- Semiconducting polymer dots for multifunctional integrated nanomedicine carriers.Materials today. Bio · 2024Review
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Advanced imaging techniques for tracking drug dynamics at the subcellular level.Advanced drug delivery reviews · 2023Review
- Dense 4D nanoscale reconstruction of living brain tissue.Nature methods · 2023Article
- Population Control of Upconversion Energy Transfer for Stimulation Emission Depletion Nanoscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Deep learning enables fast, gentle STED microscopy.Communications biology · 2023Article
- Identifying STEDable BFMolecules (Basel, Switzerland) · 2023Article
- Deep learning enables fast, gentle STED microscopy.bioRxiv : the preprint server for biology · 2023Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Super-resolution fluorescence microscopy has become an important catalyst for discovery in the life sciences. In STimulated Emission Depletion (STED) microscopy, a pattern of light drives fluorophores from a signal-emitting on-state to a non-signalling off-state. Only emitters residing in a sub-diffraction volume around an intensity minimum are allowed to fluoresce, rendering them distinguishable from the nearby, but dark fluorophores. STED routinely achieves resolution in the few tens of nanometers range in biological samples and is suitable for live imaging. Here, we review the working principle of STED and provide general guidelines for successful STED imaging. The strive for ever higher resolution comes at the cost of increased light burden. We discuss techniques to reduce light exposure and mitigate its detrimental effects on the specimen. These include specialized illumination strategies as well as protecting fluorophores from photobleaching mediated by high-intensity STED light. This opens up the prospect of volumetric imaging in living cells and tissues with diffraction-unlimited resolution in all three spatial dimensions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.