ArticleFEMS microbiology letters2019
Influence of 16S rRNA variable region on perceived diversity of marine microbial communities of the Northern North Atlantic.
Article in FEMS microbiology letters, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Standardized 16S Meta-Analysis Reveals Consistent Host-Associated Patterns in Laminariales Epiphytic Microbiomes.MicrobiologyOpen · 2026Pooled it
- Role of Core Microbiome Shifts in Octocoral Litophyton Under Diurnal Temperature Fluctuations.Microbial ecology · 2026Article
- Review
- Microbial communities associated with plastic fishing nets: diversity, potentially pathogenic and hydrocarbon degrading bacteria.Scientific reports · 2025Article
- Influence of pH and temperature on the performance and microbial community during the production of medium-chain carboxylic acids using winery effluents as substrate.Environmental science and pollution research international · 2025Article
- Improved resolution of microbial diversity in deep-sea surface sediments using PacBio long-read 16S rRNA gene sequencing.mSphere · 2024Article
- Effect of the 16S rRNA Gene Hypervariable Region on the Microbiome Taxonomic Profile and Diversity in the Endangered FishMicroorganisms · 2024Article
- Microbiome heterogeneity in tissues of the coral, Fimbriaphyllia (Euphyllia) ancora.Environmental microbiology reports · 2024Article
- Article
- Metagenomic analysis of goat feces from Ogliastra (Sardinia, Italy).Frontiers in microbiomes · 2024Article
- Selecting 16S rRNA Primers for Microbiome Analysis in a Host-Microbe System: The Case of the JellyfishMicroorganisms · 2023Article
- Paradoxical Reactions to Anti-TNFα and Anti-IL-17 Treatment in Psoriasis Patients: Are Skin and/or Gut Microbiota Involved?Dermatology and therapy · 2023Review
- Mock community as an in situ positive control for amplicon sequencing of microbiotas from the same ecosystem.Scientific reports · 2023Article
- Atlantic Salmon (Microorganisms · 2022Article
- Article
- Microbial Richness of Marine Biofilms Revealed by Sequencing Full-Length 16S rRNA Genes.Genes · 2022Article
- Microbial communities in swamps of four mangrove reserves driven by interactions between physicochemical properties and microbe in the North Beibu Gulf, China.Environmental science and pollution research international · 2022Article
- Highly-resolved interannual phytoplankton community dynamics of the coastal Northwest Atlantic.ISME communications · 2022Article
- Composition and Biogeography of Planktonic Pro- and Eukaryotic Communities in the Atlantic Ocean: Primer Choice Matters.Frontiers in microbiology · 2022Article
- Interspecies Regulation BetweenFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine microbes play essential roles in global energy and nutrient cycles. A primary method of determining their diversity and distribution is through sequencing of 16S ribosomal RNA genes from environmental samples. However, the perceived community composition may vary significantly based on differences in methodology, including choice of 16S variable region(s). This study investigated the influence of 16S variable region selection (V4-V5 or V6-V8) on perceived community composition and diversity for bacteria, Archaea and chloroplasts by tag-Illumina sequencing. We used 24 samples from the photic zone of the Scotian Shelf, northwest Atlantic, collected during a spring phytoplankton bloom. Taxonomic assignment and community composition varied greatly depending on the choice of variable regions while observed patterns of beta diversity were reproducible between variable regions. V4-V5 was considered the preferred variable region for future studies based on its superior recognition of Archaea, which has received little attention in bloom dynamics. The V6-V8 region captured more of the bacterial diversity, including the abundant SAR11 clades and, to a lesser extent, that of chloroplasts. However, the magnitude of difference between variable regions for bacteria and chloroplast was less than for Archaea.
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