Evidence map›Paper›PMID 31343579›Full record

ArticleTransplantation2019

The Effects of an IL-21 Receptor Antagonist on the Alloimmune Response in a Humanized Mouse Skin Transplant Model.

Kitty de Leur, Franka Luk, Thierry P P van den Bosch, Marjolein Dieterich, Luc J W van der Laan, Rudi W Hendriks, Marian C Clahsen-van Groningen, Fadi Issa, Carla C Baan, Martin J Hoogduijn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Transplantation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Review
  3. Interactions of the Immune System with Human Kidney Organoids.Transplant international : official journal of the European Society for Organ Transplantation · 2024
    Article
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kitty de LeurThe Rotterdam Transplant Group, Erasmus MC, Department of Internal Medicine, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Franka LukThe Rotterdam Transplant Group, Erasmus MC, Department of Internal Medicine, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Thierry P P van den BoschDepartment of Pathology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Marjolein DieterichThe Rotterdam Transplant Group, Erasmus MC, Department of Internal Medicine, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Luc J W van der LaanDepartment of Surgery, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Rudi W HendriksDepartment of Pulmonary Medicine, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Marian C Clahsen-van GroningenDepartment of Pathology, Erasmus MC, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Fadi IssaNuffield Department of Surgical Sciences, Transplantation Research Immunology Group, University of Oxford, Oxford, United Kingdom.
Carla C BaanThe Rotterdam Transplant Group, Erasmus MC, Department of Internal Medicine, University Medical Center Rotterdam, Rotterdam, The Netherlands.
Martin J HoogduijnThe Rotterdam Transplant Group, Erasmus MC, Department of Internal Medicine, University Medical Center Rotterdam, Rotterdam, The Netherlands.

Funding

Wellcome Trust 211122Wellcome Trust 211122/Z/18/Z
6 · The paper itself

Abstract

backgroundInterleukin 21 (IL-21) is involved in regulating the expansion and effector function of a broad range of leukocytes, including T cells and B cells. In transplantation, the exact role of IL-21 in the process of allograft rejection is unknown. To further explore this, the aim of this study is to test the effect of an IL-21 receptor (IL-21R) blocking antibody on the early phase of allograft rejection in a humanized skin transplantation model in mice reconstituted with human T and B cells.

methodsImmunodeficient Balb/c IL2rγRag2 mice were transplanted with human skin followed by adoptive transfer of human allogeneic splenocytes. Control animals were treated with a phosphate buffered saline vehicle while the other group was treated with a humanized anti-IL-21R antibody (αIL-21R).

resultsIn the phosphate buffered saline-treated animals, human skin allografts were infiltrated with lymphocytes and developed a thickened epidermis with increased expression of the inflammatory markers Keratin 17 (Ker17) and Ki67. In mice treated with αIL-21R, these signs of allograft reactivity were significantly reduced. Concordantly, STAT3 phosphorylation was inhibited in this group. Of note, treatment with αIL-21R attenuated the process of T and B cell reconstitution after adoptive cellular transfer.

conclusionsThese findings demonstrate that blockade of IL-21 signaling can delay allograft rejection in a humanized skin transplantation model.

Indexed as

AllograftsAnimalsB-LymphocytesDNA-Binding ProteinsGraft RejectionHumansImmunosuppressive AgentsInterleukin-21 Receptor alpha SubunitInterleukin Receptor Common gamma SubunitMice, KnockoutSkinSkin TransplantationT-LymphocytesTransplantation ChimeraTransplantation, HomologousDNA-Binding ProteinsIL21R protein, humanIl2rg protein, mouseImmunosuppressive AgentsInterleukin-21 Receptor alpha SubunitInterleukin Receptor Common gamma SubunitRag2 protein, mouse

Identifiers

PMID31343579
PMCPMC7613120

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.