Evidence map›Paper›PMID 31342207›Full record

ArticleAMB Express2019

Discovery of the Agrobacterium growth inhibition sequence in virus and its application to recombinant clone screening.

Jinlong Yin, Hui Liu, Wenyang Xiang, Tongtong Jin, Dongquan Guo, Liqun Wang, Haijian Zhi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. International journal of molecular sciences · 2023
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jinlong YinNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China.
Hui LiuNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China.
Wenyang XiangNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China.
Tongtong JinNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China.
Dongquan GuoJilin Academy of Agricultural Sciences, Changchun, 130033, People's Republic of China.
Liqun WangNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China. wanglq1124@126.com.
Haijian ZhiNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Weigang 1, Nanjing, 210095, People's Republic of China. zhj@njau.edu.cn.
Nanjing Agricultural University · CNJilin Academy of Agricultural Sciences · CN

Funding

Fund of Transgenic Breeding for Soybean Resistance to Soybean mosaic virus 2016ZX08004-004Jiangsu Collaborative Innovation Center for Modern Crop Production JCIC-MCPNational Key R&D Program of China 2017YFD0101501National Natural Science Foundation of China 31571690National Soybean Industrial Technology System of China CARS-004Research and Innovative Technology Administration (US) PCSIRT_17R55
6 · The paper itself

Abstract

Infectious clone vectors used widely in genetic research. While constructing soybean mosaic virus (SMV) clone vectors, we found that transformed Agrobacterium grew significantly different depending on the viral strains used. In particular, the clone vectors constructed with SMV SC15 significantly suppressed the growth of Agrobacterium. Recombinant and truncated virus vector experiments showed that the polymorphism of a P1 protein coding sequence of SC15 leads to the growth inhibition of Agrobacterium. But the lack of other protein encoding sequences, except for the sequence encoding coat protein, should reduce the ability of SC15 to suppress Agrobacterium growth. A vector (pCB301-attL-SC15P) compatible with the Gateway cloning system was constructed using this Agrobacterium inhibitory sequence. The results from the LR recombination reaction with pCB301-attL-SC15P and Agrobacterium transformation showed the valuable application potential of the Agrobacterium inhibitory sequence to serve as a negative screening factor for effective recombinant clone screening in Agrobacterium.

Indexed as

AgrobacteriumClone screeningGrowth inhibitionSoybean mosaic virus

Identifiers

PMID31342207
PMCPMC6656845
OpenAlexW2963609470

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.