ArticleCommunications biology2019
A quantitative, high-throughput method identifies protein-glycan interactions via mass spectrometry.
Article in Communications biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Protein Recognition of Glycosphingolipids in Membranes: Mechanistic and Quantitative Insights.Analytical chemistry · 2025Article
- CarboGrove: a resource of glycan-binding specificities through analyzed glycan-array datasets from all platforms.Glycobiology · 2022Article
- CarbArrayART: a new software tool for carbohydrate microarray data storage, processing, presentation, and reporting.Glycobiology · 2022Article
- Approaches to Heterogeneity in Native Mass Spectrometry.Chemical reviews · 2022Review
- Glycan-mediated molecular interactions in bacterial pathogenesis.Trends in microbiology · 2022Review
- Sialic acid-containing glycolipids mediate binding and viral entry of SARS-CoV-2.Nature chemical biology · 2022Article
- Quantifying Carbohydrate-Active Enzyme Activity with Glycoprotein Substrates Using Electrospray Ionization Mass Spectrometry and Center-of-Mass Monitoring.Analytical chemistry · 2021Article
- Combined Analysis of Multiple Glycan-Array Datasets: New Explorations of Protein-Glycan Interactions.Analytical chemistry · 2021Article
- Mass Spectrometric Analysis of Antibody-Epitope Peptide Complex Dissociation: Theoretical Concept and Practical Procedure of Binding Strength Characterization.Molecules (Basel, Switzerland) · 2020Article
- A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands.Nature communications · 2020Article
- Decoding glycans: deciphering the sugary secrets to be coherent on the implication.RSC advances · 2020Review
- CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities.Analytical chemistry · 2020Article
- Advances in Tools to Determine the Glycan-Binding Specificities of Lectins and Antibodies.Molecular & cellular proteomics : MCP · 2020Review
- Sliding Window Adduct Removal Method (SWARM) for Enhanced Electrospray Ionization Mass Spectrometry Binding Data.Journal of the American Society for Mass Spectrometry · 2019Article
- Quantifying Protein-Glycan Interactions Using Native Mass Spectrometry.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycan binding by glycan-binding proteins and processing by carbohydrate-active enzymes is implicated in physiological and pathophysiological processes. Comprehensive mapping of glycan interactions is essential to understanding of glycan-mediated biology and can guide the development of new diagnostics and therapeutics. Here, we introduce the competitive universal proxy receptor assay (CUPRA), which combines electrospray ionization mass spectrometry, competitive binding and heterobifunctional glycan-based ligands to give a quantitative high-throughput method for screening glycan libraries against glycan-binding and glycan-processing proteins. Application of the assay to human (siglec-2), plant (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.