ArticleScientific reports2019
Evaluation of reference genes for real-time quantitative PCR analysis in southern corn rootworm, Diabrotica undecimpunctata howardi (Barber).
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 29 citations in OpenAlex.
- Validating the classics: Accurate reference gene panel for reliable RT-qPCR in Porifera.PloS one · 2026Article
- Prognostic significance of caspase 8 associated protein 2 (CASP8AP2) in childhood b cell acute lymphoblastic Leukemia.Discover oncology · 2025Article
- Assessment of housekeeping genes stability for gene transcription regulation analysis of Spodoptera littoralis (Lepidoptera: Noctuidae) under Spodoptera littoralis nucleopolyhedrovirus viral infection.Molecular biology reports · 2024Article
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- Stable internal reference genes for quantitative RT-PCR analyses in Rhipicephalus microplus during embryogenesis.Ticks and tick-borne diseases · 2023Article
- Evaluation and validation of reference genes for RT-qPCR gene expression in Naegleria gruberi.Scientific reports · 2023Article
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- Identifying optimal reference genes for gene expression studies in Eurasian spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae).Scientific reports · 2022Article
- Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA.RNA biology · 2021Article
- Review
- Reference Gene Selection for Normalizing Gene Expression inFrontiers in physiology · 2021Article
- Article
- Suitable reference genes for RT-qPCR analysis in Dichelops melacanthus (Hemiptera: Pentatomidae).Molecular biology reports · 2020Article
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- Selection of reference genes for normalization of RT-qPCR data in gene expression studies in Anthonomus eugenii Cano (Coleoptera: Curculionidae).Scientific reports · 2020Article
- Article
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six southern corn rootworm, Diabrotica undecimpunctata howardi (Barber), housekeeping genes (HKG), β-actin (Actin), β-tubulin (Tubulin), elongation factor 1 alpha (EF1α), glyceraldehyde-3 phosphate dehydrogenase (GAPDH), 40 S ribosomal protein S9 (RpS9) and ubiquitin-conjugating protein (Ubi), under different experimental conditions such as developmental stage, exposure of neonate and adults to dsRNA, exposure of adults to different temperatures, different 3
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