ArticleJournal of virology2019
Globoside Is Dispensable for Parvovirus B19 Entry but Essential at a Postentry Step for Productive Infection.
Article in Journal of virology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The trial behind it
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Article
- Article
- Transferrin receptor 1 binds human parvovirus B19 VP1u to facilitate entry.Nature communications · 2026Article
- Parvovirus B19 IgG-Defined Prior Exposure and Its Association with Anemia in Maintenance Hemodialysis Patients: A Cross-Sectional Comparative Study.Journal of clinical medicine · 2026Article
- Cellular determinants of parvovirus B19 susceptibility in the human placenta.PLoS pathogens · 2026Article
- Viral receptors as host-pathogen control nodes linking entry, signalling, and system-level pathogenesis.FEMS microbiology reviews · 2026Review
- Parvoviruses at the Heart: Endothelial Injury and Myocyte Lysis in Human B19V and Canine CPV-2 Infections.Current issues in molecular biology · 2025Review
- Structural Capsidomics of Single-Stranded DNA Viruses.Viruses · 2025Review
- Parvovirus B19 in Rheumatic Diseases.Microorganisms · 2024Review
- Globoside Is an Essential Intracellular Factor Required for Parvovirus B19 Endosomal Escape.Cells · 2024Article
- Structural Shifts of the Parvovirus B19 Capsid Receptor-binding Domain: A Peptide Study.Protein and peptide letters · 2024Article
- Mechanism of Antigen Presentation and Specificity of Antibody Cross-Reactivity Elicited by an Oligosaccharide-Conjugate Cancer Vaccine.Journal of the American Chemical Society · 2023Article
- Globoside and the mucosal pH mediate parvovirus B19 entry through the epithelial barrier.PLoS pathogens · 2023Article
- Transient Parvovirus B19 DNAemia After Kidney Transplantation: A 2-Sided Story.Open forum infectious diseases · 2023Article
- Identification of AXL as a co-receptor for human parvovirus B19 infection of human erythroid progenitors.Science advances · 2023Article
- Nuclear entry and egress of parvoviruses.Molecular microbiology · 2022Review
- Structural Dynamics and Activity of B19V VP1u during the pHs of Cell Entry and Endosomal Trafficking.Viruses · 2022Article
- Prevalence, Cell Tropism, and Clinical Impact of Human Parvovirus Persistence in Adenomatous, Cancerous, Inflamed, and Healthy Intestinal Mucosa.Frontiers in microbiology · 2022Article
- Microbial lectome versus host glycolipidome: How pathogens exploit glycosphingolipids to invade, dupe or kill.Frontiers in microbiology · 2022Review
- Is the ZIKV Congenital Syndrome and Microcephaly Due to Syndemism with Latent Virus Coinfection?Viruses · 2021Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Globoside (Gb4) is considered the primary receptor of parvovirus B19 (B19V); however, its expression does not correlate well with the attachment and restricted tropism of the virus. The N terminus of VP1 (VP1u) of B19V interacts with an as-yet-unknown receptor required for virus internalization. In contrast to Gb4, the VP1u cognate receptor is expressed exclusively in cells that B19V can internalize. With the aim of clarifying the role of Gb4 as a B19V receptor, we knocked out the gene B3GalNT1 coding for the enzyme globoside synthase in UT7/Epo cells. Consequently, B3GalNT1 transcripts and Gb4 became undetectable in the knockout (KO) cells without affecting cell viability and proliferation. Unexpectedly, virus attachment, internalization, and nuclear targeting were not disturbed in the KO cells. However, NS1 transcription failed, and consequently, genome replication and capsid protein expression were abrogated. The block could be circumvented by transfection with a B19V infectious clone, indicating that Gb4 is not required after the generation of viral double-stranded DNA with resolved inverted terminal repeats. While in wild-type (WT) cells, occupation of the VP1u cognate receptor with recombinant VP1u disturbed virus binding and blocked the infection, antibodies against Gb4 had no significant effect. In a mixed population of WT and KO cells, B19V selectively infected WT cells. This study demonstrates that Gb4 does not have the expected receptor function, as it is dispensable for virus entry; however, it is essential for productive infection, explaining the resistance of the rare individuals lacking Gb4 to B19V infection.
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