Evidence map›Paper›PMID 31340862›Full record

ArticleParasites & vectors2019

Detection of Wolbachia in field-collected Aedes aegypti mosquitoes in metropolitan Manila, Philippines.

Thaddeus M Carvajal, Kazuki Hashimoto, Reza Kurniawan Harnandika, Divina M Amalin, Kozo Watanabe

Abstract read
In one paragraph

Article in Parasites & vectors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  4. Review
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  6. Phylogeny ofParasite epidemiology and control · 2026
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  10. Microorganisms · 2024
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  11. RevisitingEcology and evolution · 2024
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  12. Article
  13. Detection and quantification of naturalFrontiers in cellular and infection microbiology · 2024
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  14. Article
  15. Article
  16. Article
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  18. [Revista espanola de salud publica · 2023
    Article
  19. Biological comparative study betweenSaudi journal of biological sciences · 2023
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  20. Molecular Evidence ofJournal of tropical medicine · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Thaddeus M CarvajalDepartment of Civil and Environmental Engineering, Ehime University, Matsuyama, Japan.
Kazuki HashimotoDepartment of Civil and Environmental Engineering, Ehime University, Matsuyama, Japan.
Reza Kurniawan HarnandikaDepartment of Civil and Environmental Engineering, Ehime University, Matsuyama, Japan.
Divina M AmalinBiology Department, De La Salle University, Taft Avenue, Manila, Philippines.
Kozo WatanabeDepartment of Civil and Environmental Engineering, Ehime University, Matsuyama, Japan. watanabe_kozo@cee.ehime-u.ac.jp.

Funding

Japanese Society for the Promotion of Science 16H05750Japanese Society for the Promotion of Science 17H01624Japanese Society for the Promotion of Science 17K18906Japanese Society for the Promotion of Science Bilateral Joint Research ProjectsJapanese Society for the Promotion of Science Core-to-Core program (Asia-Africa Science Platforms)Leading Academia in Marine, Environmental Pollution Research Y29-1-8
6 · The paper itself

Abstract

backgroundRecent reports reveal the presence of Wolbachia in Ae. aegypti. Our study presents additional support for Wolbachia infection in Ae. aegypti by screening field-collected adult mosquitoes using two Wolbachia-specific molecular makers.

methodsA total of 672 Ae. aegypti adult mosquitoes were collected from May 2014 to January 2015 in Metropolitan Manila. Each individual sample was processed and screened for the presence of Wolbachia by selected markers, Wolbachia-specific 16S rDNA and its surface protein (wsp), under optimized PCR conditions and sequenced.

resultsTotals of 113 (16.8%) and 89 (13.2%) individual mosquito samples were determined to be infected with Wolbachia using the wsp and 16S rDNA markers, respectively. The Ae. aegpyti wsp sample sequences were similar or identical to five known Wolbachia strains belonging to supergroups A and B while the majority of 16S rDNA sample sequences were similar to strains belonging to supergroup B. Overall, 80 (11.90%) individual mosquito samples showed positive amplifications in both markers and 69% showed congruence in supergroup identification (supergroup B).

conclusionsBy utilizing two Wolbachia-specific molecular makers, our study demonstrated the presence of Wolbachia from individual Ae. aegypti samples. Our results showed a low Wolbachia infection rate and inferred the detected strains belong to either supergroups A and B.

Indexed as

AedesAnimalsDNA, RibosomalFemaleMaleMosquito VectorsPhilippinesPhylogenyWolbachiaDNA, RibosomalAe. aegyptiDengueMetropolitan ManilaWolbachia

Identifiers

PMID31340862
PMCPMC6657204

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.