Evidence map›Paper›PMID 31340803›Full record

ArticleBMC oral health2019

Gelatinolytic activity in gingival crevicular fluid and saliva of growing patients with Marfan syndrome: a case-control study.

Giuseppina Laganà, Giovanni Francesco Fasciglione, Andrea Biondi, Massimiliano Coletta, Giovanni Ruvolo, Paola Cozza

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 2 pooled it
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 2 syntheses or guidelines pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Oral Health Status in Marfan Syndrome: A Systematic Review and Meta-Analysis of 353 Cases.International journal of environmental research and public health · 2022
    Pooled it
  3. Review
  4. Saliva Analysis of pH and Antioxidant Capacity in Adult Obstructive Sleep Apnea Patients.International journal of environmental research and public health · 2022
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Giuseppina LaganàDepartment of Clinical Sciences and Translational Medicine, University of Rome, Tor Vergata Via Montpellier 1, 00133, Rome, Italy. giuseppinalagana@libero.it.ORCID 0000-0003-1841-1046
Giovanni Francesco FasciglioneDepartment of Cardiac Surgery Unit Chair Centre for Rare Diseases for Marfan Syndrome and Related Disorders, University of Tor Vergata General Hospital, Viale Oxford, 81 00133, Rome, Italy.
Andrea BiondiDepartment of Clinical Sciences and Translational Medicine, University of Rome, Tor Vergata Via Montpellier 1, 00133, Rome, Italy.
Massimiliano ColettaDepartment of Clinical Sciences and Translational Medicine, University of Rome, Tor Vergata Via Montpellier 1, 00133, Rome, Italy.
Giovanni RuvoloDepartment of Cardiac Surgery Unit Chair Centre for Rare Diseases for Marfan Syndrome and Related Disorders, University of Tor Vergata General Hospital, Viale Oxford, 81 00133, Rome, Italy.
Paola CozzaDepartment of Clinical Sciences and Translational Medicine, University of Rome, Tor Vergata Via Montpellier 1, 00133, Rome, Italy.
University of Rome Tor Vergata · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAim of the study was to evaluate the gelatinolytic activity in the saliva and gingival crevicular fluid from a sample group of subjects with Marfan syndrome.

methodsTwo groups were analyzed in this case-control study. A group of 28 subjects with Marfan syndrome (MG) was recruited from the Centre for Rare Disease, Marfan Clinic of Tor Vergata University Hospital. The second sample, 23 subjects, with the same characteristics and without any syndrome, was the control group (CG). Saliva and gingival crevicular fluid were collected and transferred to a sterile test tube and stored frozen at - 20 °C until analysis at the Medical Chemistry Laboratory. Gelatin substrate zymography was used for the evaluation and characterization of saliva and crevicular fluid proteinases. Correlation test and Student's t-test have been used to analyze data.

resultsIn all samples different gelatin-degrading activities were observed. Two bands, which are related to the molecular weights of pro-MMP-9 and active MMP-9, respectively, were detectable in 100% of Marfan and control samples. MMP-2 activity was higher in Marfan group. Additional bands (55/48 kDa), corresponding to the activated forms of collagenase (MMP-13), were observed in saliva samples of both groups.

conclusionsThe association of an enhanced activity by MMP-13 with an increased amount of active MMP-9 might be an important biomarker for the diagnosis of Marfan syndrome.

Indexed as

Case-Control StudiesChildFemaleGelatinGingival Crevicular FluidHumansMaleMarfan SyndromeMatrix Metalloproteinase 13Matrix Metalloproteinase 2SalivaGelatinMatrix Metalloproteinase 13Matrix Metalloproteinase 2MMP13 protein, humanMMP2 protein, humanCrevicular fluidGelatinolytic activityMarfan syndromeMMPSaliva

Identifiers

PMID31340803
PMCPMC6657031
OpenAlexW2962936703

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.