ArticlePloS one2019
Gaining ecological insight on dietary allocation among horseshoe bats through molecular primer combination.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 39 citations in OpenAlex.
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- Moth munchers: limited intraspecific variation in summer diet of the endangered Florida Bonneted Bat (Journal of mammalogy · 2025Article
- DNA metabarcoding analysis of stomach flushing contents reveals the exceptionally diverse diet of the golden alpine salamander.Scientific reports · 2025Article
- Metabarcoding provides accurate estimation of volumetric diet composition in a top predator despite interference from blocking primers.Scientific reports · 2025Article
- Learning to Hunt on the Go: Dietary Changes During Development of Rhinolophid Bats.Animals : an open access journal from MDPI · 2024Article
- Aerospace-foraging bats eat seasonably across varying habitats.Scientific reports · 2023Article
- DNA metabarcoding suggests adaptive seasonal variation of individual trophic traits in a critically endangered fish.Molecular ecology · 2022Article
- Dietary preferences and feeding strategies of Colombian highland woolly monkeys.Scientific reports · 2022Article
- DNA metabarcoding quantifies the relative biomass of arthropod taxa in songbird diets: Validation with camera-recorded diets.Ecology and evolution · 2022Article
- Molecular assays to reliably detect and quantify predation on a forest pest in bats faeces.Scientific reports · 2022Article
- Biological composition analysis of a natural medicine, Faeces Vespertilionis, with complex sources using DNA metabarcoding.Scientific reports · 2022Article
- Do the evolutionary interactions between moths and bats promote niche partitioning between bats and birds?Ecology and evolution · 2021Article
- Nontuberculous Mycobacteria Prevalence in Bats' Guano from Caves and Attics of Buildings Studied by Culture and qPCR Examinations.Microorganisms · 2021Article
- Food for everyone: Differential feeding habits of cryptic bat species inferred from DNA metabarcoding.Molecular ecology · 2021Article
- Perch time allocation and feeding efficiency of flycatching Rhinolophus formosae: an optimal foraging behavior?BMC zoology · 2021Article
- Body Size Variation in Italian Lesser Horseshoe BatsBiology · 2020Article
- Trophic resource partitioning drives fine-scale coexistence in cryptic bat species.Ecology and evolution · 2020Article
- DNA metabarcoding and spatial modelling link diet diversification with distribution homogeneity in European bats.Nature communications · 2020Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge on the trophic interactions among predators and their prey is important in order to understand ecology and behaviour of animals. Traditionally studies on the diet composition of insectivorous bats have been based on the morphological identification of prey remains, but the accuracy of the results has been hampered due to methodological limitations. Lately, the DNA metabarcoding and High Throughput Sequencing (HTS) techniques have changed the scene since they allows prey identification to the species level, ultimately giving more precision to the results. Nevertheless, the use of one single primer set to amplify faecal DNA produces biases in the assessed dietary composition. Three horseshoe bats overlap extensively in their distribution range in Europe: Rhinolophus euryale, R. hipposideros and R. ferrumequinum. In order to achieve the deepest insight on their prey list we combined two different primers. Results showed that the used primers were complementary at the order and species levels, only 22 out of 135 prey species being amplified by both. The most frequent prey of R. hipposideros belonged to Diptera and Lepidoptera, to Lepidoptera in R. euryale, and Lepidoptera, Diptera and Coleoptera in R. ferrumequinum. The three bats show significant resource partitioning, since their trophic niche overlap is not higher than 34%. Our results confirm the importance of combining complementary primers to describe the diet of generalist insectivorous bats with amplicon metabarcoding techniques. Overall, each primer set showed a subset of the prey composition, with a small portion of the total prey being identified by both of them. Therefore, each primer presented a different picture of the niche overlap among the three horseshoe bats due to their taxonomic affinity.
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