ArticleNature communications2019
A standard for near-scarless plasmid construction using reusable DNA parts.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- A microbial platform for selective biosynthesis of monoterpene esters.Nature communications · 2026Article
- A modular single- and dual-gene expression toolkit for Kluyveromyces marxianus.World journal of microbiology & biotechnology · 2026Article
- Blended nexus molecules promote COScience advances · 2024Article
- SEVA 4.0: an update of the Standard European Vector Architecture database for advanced analysis and programming of bacterial phenotypes.Nucleic acids research · 2023Article
- Biosynthesis of geranate via isopentenol utilization pathway in Escherichia coli.Biotechnology and bioengineering · 2023Article
- Single 3'-exonuclease-based multifragment DNA assembly method (SENAX).Scientific reports · 2022Article
- A Flagella Hook Coding GeneFrontiers in microbiology · 2022Article
- Improving protein solubility and activity by introducing small peptide tags designed with machine learning models.Metabolic engineering communications · 2020Article
- Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture ofMetabolic engineering communications · 2020Article
- Rapid, robust plasmid verification by de novo assembly of short sequencing reads.Nucleic acids research · 2020Article
- Modular engineering for microbial production of carotenoids.Metabolic engineering communications · 2020Review
- Upcycling chitin-containing waste into organonitrogen chemicals via an integrated process.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we report GT (Guanin/Thymine) standard (GTS) for plasmid construction under which DNA sequences are defined as two types of standard, reusable parts (fragment and barcode). We develop a technology that can efficiently add any two barcodes to two ends of any fragment without leaving scars in most cases. We can assemble up to seven such barcoded fragments into one plasmid by using one of the existing DNA assembly methods, including CLIVA, Gibson assembly, In-fusion cloning, and restriction enzyme-based methods. Plasmids constructed under GTS can be easily edited, and/or be further assembled into more complex plasmids by using standard DNA oligonucleotides (oligos). Based on 436 plasmids we constructed under GTS, the averaged accuracy of the workflow was 85.9%. GTS can also construct a library of plasmids from a set of fragments and barcodes combinatorically, which has been demonstrated to be useful for optimizing metabolic pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.