ArticleProceedings of the National Academy of Sciences of the United States of America2019
A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Sprint interval exercise disrupts mitochondrial ultrastructure driving a unique mitochondrial stress response and remodelling in men.Nature communications · 2025Trial
- Substrate-Controlled Enantiodivergence in Ni-Catalyzed Access to Phosphorylated Oxindoles With Quaternary Stereocenters.Angewandte Chemie (International ed. in English) · 2026Article
- Organelle-specific fluorescent probes for live imaging at the nanoscale.Science China. Life sciences · 2026Review
- A Molecular Engineering Strategy to Fine-Tune Phototoxicity of AIE Probes for Super-Resolution Imaging of Mitochondrial Cristae Dynamics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- From Microscopy to Nanoscopy: Contemporary Physical Methods in Mitochondrial Structural Biology.International journal of molecular sciences · 2026Review
- High-content phenotyping reveals Golgi dynamics and their role in cell cycle regulation.The Journal of cell biology · 2026Article
- A neural network for long-term super-resolution imaging of live cells with reliable confidence quantification.Nature biotechnology · 2026Article
- There and back again: a cell biologist's journey from organelles to molecules.Biological chemistry · 2025Review
- Illuminating Mitochondrial Dynamics: Ultrahigh Labeling Stability Probe for Long-Term SIM Super-Resolution Imaging of Mitochondria.ACS central science · 2025Article
- Molecular machineries shaping the mitochondrial inner membrane.Nature reviews. Molecular cell biology · 2025Review
- Phosphole-Based Fluorescent Biomaterials for Imaging and Therapy.Chemical & biomedical imaging · 2025Review
- A data-driven model for mitochondrial inner membrane remodeling as a driving force of organelle shaping.Journal of cell science · 2025Article
- Copper Chelation Induces Morphology Change in Mitochondria of Triple-Negative Breast Cancer.JACS Au · 2025Article
- Glycocalyx-induced formation of membrane tubes.Biophysical journal · 2025Article
- Captodative Approach to Stable Nitrogen-Centered Radicals, Anions, and Cations Exhibiting Near-Infrared Electrochromism.JACS Au · 2025Article
- Fluorescent probes for the visualization of membrane microdomain, deformation, and fusion.Smart molecules : open access · 2025Review
- DeepCristae, a CNN for the restoration of mitochondria cristae in live microscopy images.Communications biology · 2025Article
- Review
- Fortunate molecules boost signal to background ratio and localization precision in correlation based single molecule localization microscopy.Communications biology · 2024Article
- Visualizing mitochondrial dynamics at the nanoscale.Light, science & applications · 2024Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Stimulation emission depletion (STED) microscopy enables ultrastructural imaging of organelle dynamics with a high spatiotemporal resolution in living cells. For the visualization of the mitochondrial membrane dynamics in STED microscopy, rationally designed mitochondrial fluorescent markers with enhanced photostability are required. Herein, we report the development of a superphotostable fluorescent labeling reagent with long fluorescence lifetime, whose design is based on a structurally reinforced naphthophosphole fluorophore that is conjugated with an electron-donating diphenylamino group. The combination of long-lived fluorescence and superphotostable features of the fluorophore allowed us to selectively capture the ultrastructures of the mitochondrial cristae with a resolution of ∼60 nm when depleted at 660 nm. This chemical tool provides morphological information of the cristae, which has so far only been observed in fixed cells using electron microscopy. Moreover, this method gives information about the dynamic ultrastructures such as the intermembrane fusion in different mitochondria as well as the intercristae mergence in a single mitochondrion during the apoptosis-like mitochondrial swelling process.
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