ArticleProceedings of the National Academy of Sciences of the United States of America2019
Methodology and theoretical basis of forward genetic screening for sleep/wakefulness in mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 40 citations in OpenAlex.
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- SIK3-HDAC4 in the suprachiasmatic nucleus regulates the timing of arousal at the dark onset and circadian period in mice.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Home-cage behavior in the Stargazer mutant mouse.Scientific reports · 2022Article
- Characterization of single nucleotide polymorphisms for a forward genetics approach using genetic crosses in C57BL/6 and BALB/c substrains of mice.Experimental animals · 2022Article
- Translational relevance of forward genetic screens in animal models for the study of psychiatric disease.Neuroscience and biobehavioral reviews · 2022Review
- Mice Lacking Cerebellar Cortex and Related Structures Show a Decrease in Slow-Wave Activity With Normal Non-REM Sleep Amount and Sleep Homeostasis.Frontiers in behavioral neuroscience · 2022Article
- Protocol for sleep analysis in the brain of genetically modified adult mice.STAR protocols · 2021Article
- Two novel mouse models mimicking minor deletions in 22q11.2 deletion syndrome revealed the contribution of each deleted region to psychiatric disorders.Molecular brain · 2021Article
- Induction of MutantThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- The Role of Reproductive Hormones in Sex Differences in Sleep Homeostasis and Arousal Response in Mice.Frontiers in neuroscience · 2021Article
- Gut microbiota depletion by chronic antibiotic treatment alters the sleep/wake architecture and sleep EEG power spectra in mice.Scientific reports · 2020Article
- A most formidable arsenal: genetic technologies for building a better mouse.Genes & development · 2020Review
- Facilitating Complex Trait Analysis via Reduced Complexity Crosses.Trends in genetics : TIG · 2020Review
- Forward genetic analysis using OCT screening identifiesProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Recent advances in understanding the genetics of sleep.F1000Research · 2020Review
- Forward genetic approach for behavioral neuroscience using animal models.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2020Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulatory network of genes and molecules in sleep/wakefulness remains to be elucidated. Here we describe the methodology and workflow of the dominant screening of randomly mutagenized mice and discuss theoretical basis of forward genetics research for sleep in mice. Our high-throughput screening employs electroencephalogram (EEG) and electromyogram (EMG) to stage vigilance states into a wake, rapid eye movement sleep (REMS) and non-REM sleep (NREMS). Based on their near-identical sleep/wake behavior, C57BL/6J (B6J) and C57BL/6N (B6N) are chosen as mutagenized and counter strains, respectively. The total time spent in the wake and NREMS, as well as the REMS episode duration, shows sufficient reproducibility with small coefficients of variance, indicating that these parameters are most suitable for quantitative phenotype-driven screening. Coarse linkage analysis of the quantitative trait, combined with whole-exome sequencing, can identify the gene mutation associated with sleep abnormality. Our simulations calculate the achievable LOD score as a function of the phenotype strength and the numbers of mice examined. A pedigree showing a mild decrease in total wake time resulting from a heterozygous point mutation in the
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