Evidence map›Paper›PMID 31336721›Full record

ArticleDiagnostics (Basel, Switzerland)2019

Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor.

Flora M Yrad, Josephine M Castañares, Evangelyn C Alocilja

Abstract read
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Biosensors for the detection of flaviviruses: A review.Synthetic and systems biotechnology · 2025
    Review
  8. Article
  9. Article
  10. Review
  11. Rapid detection ofMicrobiology spectrum · 2024
    Article
  12. Review
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Detection of UnamplifiedBiosensors · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Flora M YradNano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA.ORCID 0000-0002-3916-3427
Josephine M CastañaresDepartment of Chemistry, University of San Carlos, Talamban, Cebu 6000, Philippines.
Evangelyn C AlociljaNano-Biosensors Lab, Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI 48824, USA. alocilja@msu.edu.

Funding

United States Agency for International Development 155588
6 · The paper itself

Abstract

Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. Proof-of-concept application of the biosensor detected dengue-1 virus in pooled human sera with a cut-off value of 1.2 × 10

Indexed as

dengue-1dextrin-capped AuNPlateral flow biosensorNASBAsandwich-type hybridization

Identifiers

PMID31336721
PMCPMC6787709

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.