Evidence map›Paper›PMID 31336696›Full record

ArticleMolecules (Basel, Switzerland)2019

A Novel 3-Phytosterone-9α-Hydroxylase Oxygenation Component and Its Application in Bioconversion of 4-Androstene-3,17-Dione to 9α-Hydroxy-4-Androstene-3,17-Dione Coupling with A NADH Regeneration Formate Dehydrogenase.

Xian Zhang, Manchi Zhu, Rumeng Han, Youxi Zhao, Kewei Chen, Kai Qian, Minglong Shao, Taowei Yang, Meijuan Xu, Jianzhong Xu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Xian ZhangThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.ORCID 0000-0002-1689-3232
Manchi ZhuThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.
Rumeng HanThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.
Youxi ZhaoBiochemical Engineering College, Beijing Union University, Beijing 100023, China.
Kewei ChenSchool of Food Science and Technology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.
Kai QianSchool of Medicine, Yichun University, Yichun 336000, Jiangxi, China.
Minglong ShaoThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.
Taowei YangThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.
Meijuan XuThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China.
Jianzhong XuThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China. xujianzhong@jiangnan.edu.cn.ORCID 0000-0003-0750-6875
Zhiming RaoThe Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Liu Avenue, Wuxi 214122, Jiangsu, China. raozhm@jiangnan.edu.cn.
Jiangnan University · CNBeijing Union University · CNYichun University · CN

Funding

Key research and development program of Ningxia hui autonomous region 2017BY069national first-class discipline program of Light Industry Technology and Engineering LITE2018-06National Natural Science Foundation of China 31500065, 21778024The science and technology innovation team foundation of Ningxia hui autonomous region KJT2017001
6 · The paper itself

Abstract

9α-Hydroxy-4-androstene-3,17-dione (9-OH-AD) is one of the significant intermediates for the preparation of β-methasone, dexamethasone, and other steroids. In general, the key enzyme that enables the biotransformation of 4-androstene-3,17-dione (AD) to 9-OH-AD is 3-phytosterone-9α-hydroxylase (KSH), which consists of two components: a terminal oxygenase (KshA) and ferredoxin reductase (KshB). The reaction is carried out with the concomitant oxidation of NADH to NAD

Indexed as

BiotransformationOxidation-ReductionAndrostenesBacterial ProteinsBiocatalysisEscherichia coliFormate DehydrogenasesHydroxylationMetabolic Networks and PathwaysMixed Function OxygenasesNADAndrostenesBacterial ProteinsFormate DehydrogenasesMixed Function OxygenasesNAD3-phytosterone-9α-hydroxylase9α-Hydroxy-4-androstene-3,17-dionehydroxylationNADH regeneration

Identifiers

PMID31336696
PMCPMC6680482
OpenAlexW2961995133

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.