ArticleThe Journal of clinical investigation1988
Human eosinophils have prolonged survival, enhanced functional properties, and become hypodense when exposed to human interleukin 3.
Article in The Journal of clinical investigation, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
76 citing papers in PubMed, 476 citations in OpenAlex.
- Rediscovering eosinophil identity through tissue adaptation.Nature immunology · 2026Review
- Fueling the Seed: Growth Factors and Cytokines Driving Cancer Stem Cells in Gynecological Malignancies.International journal of molecular sciences · 2025Review
- IL-3: key orchestrator of inflammation.Frontiers in immunology · 2024Review
- Transcriptomic analysis of the anti-inflammatory effect ofFrontiers in pharmacology · 2022Article
- Review
- Assessment of faithful interleukin-3 production by novel bicistronic interleukin-3 reporter mice.Immunology letters · 2020Article
- Innate and secondary humoral responses are improved by increasing the time between MVA vaccine immunizations.NPJ vaccines · 2020Article
- IL-4 enhances survival of in vitro-differentiated mouse basophils through transcription-independent signaling downstream of PI3K.Cell death & disease · 2018Article
- Article
- Modeling TImmunological reviews · 2017Review
- Activated Stat5 trafficking Via Endothelial Cell-derived Extracellular Vesicles Controls IL-3 Pro-angiogenic Paracrine Action.Scientific reports · 2016Article
- Grasping nettles: cellular heterogeneity and other confounders in epigenome-wide association studies.Human molecular genetics · 2014Review
- CD4+ T cells enhance the unloaded shortening velocity of airway smooth muscle by altering the contractile protein expression.The Journal of physiology · 2014Article
- Interleukin-3-deficient mice have increased resistance to blood-stage malaria.Infection and immunity · 2014Article
- CD 123 is a membrane biomarker and a therapeutic target in hematologic malignancies.Biomarker research · 2014Article
- Eosinophil cytokines, chemokines, and growth factors: emerging roles in immunity.Frontiers in immunology · 2014Review
- Biomarkers of eosinophil involvement in allergic and eosinophilic diseases: review of phenotypic and serum markers including a novel assay to quantify levels of soluble Siglec-8.Journal of immunological methods · 2012Article
- IL-3 and TNFα increase Thymic Stromal Lymphopoietin Receptor (TSLPR) expression on eosinophils and enhance TSLP-stimulated degranulation.Clinical and molecular allergy : CMA · 2012Article
- Eosinophils and disease pathogenesis.Seminars in hematology · 2012Review
- Treatment of allergic asthma: modulation of Th2 cells and their responses.Respiratory research · 2011Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Human eosinophils were cultured in the presence of recombinant human IL-3 for up to 14 d and their biochemical, functional, and density properties were assessed. After 3 d of culture in 10 pM IL-3, eosinophils had a viability of 70% compared with only 10% in enriched medium alone. Neither IL-1 alpha, IL-2, IL-4, tumor necrosis factor, basic fibroblast growth factor, nor platelet-derived growth factor maintained eosinophil viability. The 7- and 14-d survival of the cultured eosinophils was 55 and 53%, respectively. No other cell type, including neutrophils, was present after culture. After 7 d of culture, the normodense eosinophils were converted to hypodense cells as assessed by density centrifugation. Eosinophils exposed to 1,000 pM IL-3 for 30 min or cultured in 10 pM IL-3 for 7 d generated approximately threefold more leukotriene C4 (LTC4) in response to calcium ionophore than freshly isolated cells. Furthermore, whereas freshly isolated eosinophils killed only 14% of the antibody-coated Schistosoma mansoni larvae, these eosinophils killed 54% of the larvae when exposed to 100 pM IL-3. The enhanced helminth cytotoxicity was maintained for 7 d when eosinophils were cultured in the presence of both 10 pM IL-3 and 3T3 fibroblasts, but not when eosinophils were cultured in the presence of IL-3 alone. IL-3 thus maintains the viability of eosinophils in vitro, augments the calcium ionophore-induced generation of LTC4, enhances cytotoxicity against antibody-sensitized helminths, and induces the eosinophils to become hypodense cells. These phenotypic changes in the eosinophil may be advantageous to host defense against helminthic infections but may be disadvantageous in conditions such as allergic disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.