ArticleJournal of biological engineering2019
Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates.
Article in Journal of biological engineering, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
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- Article
- Evolved DNAzymes and Stable Activation Chemistry Enable High-Efficiency DNA Ligation.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Protein nanocages: A new frontier in mucosal vaccine delivery and immune activation.Human vaccines & immunotherapeutics · 2025Review
- Bionanoconjugates in Neurodegeneration: Peptide-Nanoparticle Alliances for Next-Generation Therapies.Pharmaceutical research · 2025Review
- Insulin Quantification Through Electrochemical and Optical Aptasensors: A Review.Annals of biomedical engineering · 2025Review
- Nanostructured lipid carriers for enhanced batimastat delivery across the blood-brain barrier: an in vitro study for glioblastoma treatment.Drug delivery and translational research · 2025Article
- Innovative Use of Gallic Acid as a Crosslinking Agent for Gelatin: A Biocompatible Strategy for 3D-Printed Scaffolds in Tissue Engineering.Pharmaceutics · 2025Article
- Covalent Peptide-Graphene Conjugates for Enhanced Cell Spreading, Osteogenic Differentiation, and Angiogenesis in Bone Defects.Chembiochem : a European journal of chemical biology · 2025Article
- Surface Modification, Toxicity, and Applications of Carbon Dots to Cancer Theranosis: A Review.Nanomaterials (Basel, Switzerland) · 2025Review
- Rhamnolipid-Modified PHB-Ectoine Nanoparticles for Multifunctional Skin Protection Against UVB, Irritation, and Bacteria.ACS omega · 2025Article
- PARPs and ADP-ribosylation-mediated biomolecular condensates: determinants, dynamics, and disease implications.Trends in biochemical sciences · 2025Review
- Interaction of Nanomaterials with Nucleic Acids and Their Applications in Nucleic Acid Analysis.International journal of biological sciences · 2025Review
- The Effect of Carbodiimide Crosslinkers on Gelatin Hydrogel as a Potential Biomaterial for Gingival Tissue Regeneration.Gels (Basel, Switzerland) · 2024Article
- Bioconjugation of Serratiopeptidase with Titanium Oxide Nanoparticles: Improving Stability and Antibacterial Properties.Journal of functional biomaterials · 2024Article
- Cross-Linking Agents in Three-Component Materials Dedicated to Biomedical Applications: A Review.Polymers · 2024Review
- Synthesis and characterization of amine-functionalized graphene as a nitric oxide-generating coating for vascular stents.Applied physics reviews · 2024Article
- Acidochromic Free-Standing Multilayered Chitosan-Pyranoflavylium/Alginate Membranes toward Food Smart Packaging Applications.ACS applied polymer materials · 2024Article
- Quantum Dot-based Bio-conjugates as an Emerging Bioimaging Tool for Cancer Theranostic- A Review.Current drug targets · 2024Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An adapted strategy from the conventional 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) crosslinking method was developed to form a covalently coupled phosphoramidated single stranded DNA (ssDNA). Matrix assisted laser desorption ionization-time of flight (MALDI-TOF) results demonstrated that the phosphoramidated ssDNA conjugate is stable for several days, and that phosphoramidation occurred exclusively at the 5' phosphate of ssDNA. A reversed phase high-performance liquid chromatography (RP-HPLC) method with UV detection was developed to determine the yield of conjugates. The methods coefficients of variation (%CV) were less than 6%, and biases ranged from - 5.1 - 1.2%. The conjugate yield via the conventional EDC method was 68.3 ± 2.2%, while that of the adapted EDC/Imidazole method was 79.0 ± 2.4% (
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Registered trials
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