ArticleScientific reports2019
Identification of novel breakpoints for locus- and region-specific translocations in 293 cells by molecular cytogenetics before and after irradiation.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
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- Comparative Analysis of HEK293 Genomic Variability.Biotechnology and bioengineering · 2026Article
- Investigating and correcting a rare pathogenic mutation in GDF11.HGG advances · 2026Article
- Gene-sized DNA insertion at genomic safe harbors in human cells using a site-directed transposase.Nucleic acids research · 2025Article
- Precise correction of G6PD Viangchan mutation in iPSCs by prime editing strategy.Scientific reports · 2025Article
- Precision multiplexed base editing in human cells using Cas12a-derived base editors.Nature communications · 2025Article
- G-Banding and Molecular Cytogenetics Detect Novel Translocations and Cryptic Aberrations in Human Immortal Endothelial Cells.International journal of molecular sciences · 2024Article
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- CRISPR prime editing for unconstrained correction of oncogenic KRAS variants.Communications biology · 2023Article
- Targeted assembly of ectopic kinetochores to induce chromosome-specific segmental aneuploidies.The EMBO journal · 2023Article
- Cytogenetic and epigenetic aberrations in peripheral lymphocytes of northwest Arkansas Marshallese.International journal of radiation biology · 2023Article
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- Global chromosome rearrangement induced by CRISPR-Cas9 reshapes the genome and transcriptome of human cells.Nucleic acids research · 2022Article
- Evaluating the effects of low-dose simulated galactic cosmic rays on murine hippocampal-dependent cognitive performance.Frontiers in neuroscience · 2022Article
- Novel cell lines derived from Chinese hamster kidney tissue.PloS one · 2022Article
- Dietary Methionine Deficiency Enhances Genetic Instability in Murine Immune Cells.International journal of molecular sciences · 2021Article
- Human L1 Transposition Dynamics Unraveled with Functional Data Analysis.Molecular biology and evolution · 2020Article
- Genome targeting by hybrid Flp-TAL recombinases.Scientific reports · 2020Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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Abstract
The human kidney embryonic 293 cell line (293 cells) is extensively used in biomedical and pharmaceutical research. These cells exhibit a number of numerical and structural chromosomal anomalies. However, the breakpoints responsible for these structural chromosomal rearrangements have not been comprehensively characterized. In addition, it is not known whether chromosomes with structural rearrangement are more sensitive to external toxic agents, such as ionizing radiation. We used G-banding, spectral karyotyping (SKY), and locus- and region-specific fluorescence in situ hybridization (FISH) probes designed in our lab or obtained from commercial vendor to address this gap. Our G-banding analysis revealed that the chromosome number varies from 66 to 71, with multiple rearrangements and partial additions and deletions. SKY analysis confirmed 3 consistent rearrangements, two simple and one complex in nature. Multicolor FISH analysis identified an array of breakpoints responsible for locus- and region-specific translocations. Finally, SKY analysis revealed that radio-sensitivity of structurally rearranged chromosomes is dependent on radiation dose. These findings will advance our knowledge in 293 cell biology and will enrich the understanding of radiation biology studies.
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