ArticleScientific reports2019
Vps10-mediated targeting of Pep4 determines the activity of the vacuole in a substrate-dependent manner.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Retromer Regulates Macro- and Micro-Autophagy via Distinct Vacuolar Proteases in the Rice Blast Fungus.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Vacuolar sorting receptors coordinate lytic vacuolar and autophagic transport for plant effector-triggered immunity.Nature plants · 2025Article
- Alleviating vacuolar transport improves cellulase production in Trichoderma reesei.Applied microbiology and biotechnology · 2023Article
- Molecular basis of the glycosomal targeting of PEX11 and its mislocalization to mitochondrion in trypanosomes.Frontiers in cell and developmental biology · 2023Article
- Neurodegenerative disease-associated inclusion bodies are cleared by selective autophagy in budding yeast.Autophagy reports · 2023Article
- Comparative Transcriptome Analysis Unravels the Response Mechanisms ofInternational journal of molecular sciences · 2022Article
- Autophagy Stimulus-Dependent Role of the Small GTPase Ras2 in Peroxisome Degradation.Biomolecules · 2020Article
- The Peroxisomal PTS1-Import Defect ofInternational journal of molecular sciences · 2020Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The vacuole is the hydrolytic compartment of yeast cells and has a similar function as the lysosome of higher eukaryotes in detoxification and recycling of macromolecules. We analysed the contribution of single vacuolar enzymes to pexophagy and identified the phospholipase Atg15, the V-ATPase factor Vma2 and the serine-protease Prb1 along with the already known aspartyl-protease Pep4 (Proteinase A) to be required for this pathway. We also analysed the trafficking receptor Vps10, which is required for an efficient vacuolar targeting of the precursor form of Pep4. Here we demonstrate a novel context-dependent role of Vps10 in autophagy. We show that reduced maturation of Pep4 in a VPS10-deletion strain affects the proteolytic activity of the vacuole depending on the type and amount of substrate. The VPS10-deletion has no effect on the degradation of the cytosolic protein Pgk1 via bulk autophagy or on the degradation of ribosomes via ribophagy. In contrast, the degradation of an excess of peroxisomes via pexophagy as well as mitochondria via mitophagy was significantly hampered in a VPS10-deletion strain and correlated with a decreased maturation level of Pep4. The results show that Vps10-mediated targeting of Pep4 limits the proteolytic capacity of the vacuole in a substrate-dependent manner.
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