ArticleScientific reports2019
Optical coherence tomography complements confocal microscopy for investigation of multicellular tumour spheroids.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- The autocrine loop of ALK receptor and ALKAL2 ligand is an actionable target in consensus molecular subtype 1 colon cancer.Journal of experimental & clinical cancer research : CR · 2022Pooled it
- Intrinsically co-registered extended-depth-of-focus OCM and two-photon microscopy for label-free volumetric analysis of 3D cell models.Biomedical optics express · 2026Article
- An overview of the developments in 3D cancer cell models, assay techniques, and imaging modalities.Human cell · 2026Review
- Ophthalmological manifestations in autoimmune diseases: Overcoming diagnostic and therapeutic challenges.World journal of experimental medicine · 2025Review
- Utility of Multicellular Spheroids for Investigating Mechanisms of Chemoresistance in Triple-Negative Breast Cancer.International journal of molecular sciences · 2025Review
- Imaging 3D cell cultures with optical microscopy.Nature methods · 2025Review
- Dual-parameter tomographic imaging of attenuation and backscattering coefficients for quantitative evaluation of immune cell-mediated cytotoxicity in tumor spheroids.Theranostics · 2025Article
- Ultrasound-induced reorientation for multi-angle optical coherence tomography.Nature communications · 2024Article
- Label-free visualization and quantification of the drug-type-dependent response of tumor spheroids by dynamic optical coherence tomography.Scientific reports · 2024Article
- Doxorubicin-sensitive and -resistant colorectal cancer spheroid models: assessing tumor microenvironment features for therapeutic modulation.Frontiers in cell and developmental biology · 2023Article
- Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment.Journal of visualized experiments : JoVE · 2022Article
- Review
- Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens.ACS omega · 2021Article
- Morphological Response in Cancer Spheroids for Screening Photodynamic Therapy Parameters.Frontiers in molecular biosciences · 2021Article
- The importance of scoring recognition fitness in spheroid morphological analysis for robust label-free quality evaluation.Regenerative therapy · 2020Article
- A Reliable Flow-Based Method for the Accurate Measure of Mass Density, Size and Weight of Live 3D Tumor Spheroids.Micromachines · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knowledge of optical properties, such as the refractive index (RI), of biological tissues is important in optical imaging, as they influence the distribution and propagation of light in tissue. To accurately study the response of cancerous cells to drugs, optimised imaging protocols are required. This study uses a simple custom-built spectral domain optical coherence tomography (OCT) system to conduct RI measurements of multicellular spheroids, three-dimensional (3D) in-vitro culture systems, of the cell line HCT116. The spheroid RIs are compared to study the effect of growth over time. To improve confocal microscopy imaging protocols, two immersion media (glycerol and ScaleView-A2) matching the spheroid RIs were trialled, with the aim to reduce the RI mismatch between the spheroid and the immersion medium and thus improve imaging depth with confocal microscopy. ScaleView-A2 (n = 1.380) aided in achieving greater depths of imaging of the multicellular spheroids under confocal microscopy. This improvement in imaging depth confirmed the utility of our RI measurements, proving the promising outlook of OCT as a complementary tool to microscopy in cancer research.
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