Evidence map›Paper›PMID 31331014›Full record

ArticleMicromachines2019

Micro Vacuum Chuck and Tensile Test System for Bio-Mechanical Evaluation of 3D Tissue Constructed of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes (hiPS-CM).

Kaoru Uesugi, Fumiaki Shima, Ken Fukumoto, Ayami Hiura, Yoshinari Tsukamoto, Shigeru Miyagawa, Yoshiki Sawa, Takami Akagi, Mitsuru Akashi, Keisuke Morishima

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Micromachines, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kaoru UesugiDepartment of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Fumiaki ShimaBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
Ken FukumotoBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
Ayami HiuraBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
Yoshinari TsukamotoBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
Shigeru MiyagawaGlobal Center for Medical Engineering and Informatics, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, Japan.
Yoshiki SawaGlobal Center for Medical Engineering and Informatics, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, Japan.
Takami AkagiBuilding Block Science Joint Research Chair, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, Osaka 565-0871, Japan.
Mitsuru AkashiGlobal Center for Medical Engineering and Informatics, Osaka University, 2-1 Yamada-oka Suita, Osaka 565-0871, Japan.
Keisuke MorishimaDepartment of Mechanical Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan. morishima@mech.eng.osaka-u.ac.jp.

Funding

Japan Agency for Medical Research and Development P14028
6 · The paper itself

Abstract

In this report, we propose a micro vacuum chuck (MVC) which can connect three-dimensional (3D) tissues to a tensile test system by vacuum pressure. Because the MVC fixes the 3D tissue by vacuum pressure generated on multiple vacuum holes, it is expected that the MVC can fix 3D tissue to the system easily and mitigate the damage which can happen by handling during fixing. In order to decide optimum conditions for the size of the vacuum holes and the vacuum pressure, various sized vacuum holes and vacuum pressures were applied to a normal human cardiac fibroblast 3D tissue. From the results, we confirmed that a square shape with 100 µm sides was better for fixing the 3D tissue. Then we mounted our developed MVCs on a specially developed tensile test system and measured the bio-mechanical property (beating force) of cardiac 3D tissue which was constructed of human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CM); the 3D tissue had been assembled by the layer-by-layer (LbL) method. We measured the beating force of the cardiac 3D tissue and confirmed the measured force followed the Frank-Starling relationship. This indicates that the beating property of cardiac 3D tissue obtained by the LbL method was close to that of native cardiac tissue.

Indexed as

beating forcebio-mechanical propertycardiac 3D tissuehuman induced pluripotent Stem cell-derived cardiomyocytes (hiPS-CM)tissue engineeringvacuum chuck

Identifiers

PMID31331014
PMCPMC6680730

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.