ArticleViruses2019
A Protocol for Extraction of Infective Viromes Suitable for Metagenomics Sequencing from Low Volume Fecal Samples.
Article in Viruses, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Characterization of novel mycobacteriophages with a focus on phage Pisa4 and its interaction with human innate immune cells.Frontiers in microbiology · 2026Article
- Infant gut anellovirus composition associates with early life factors and childhood atopic disease.Nature communications · 2025Article
- The influence of early life exposures on the infant gut virome.Gut microbes · 2025Article
- Prophages in the infant gut are pervasively induced and may modulate the functionality of their hosts.NPJ biofilms and microbiomes · 2025Article
- Ecological strategies of bacteria shape inherent phage diversity in Atlantic salmon gut microbiomes.The ISME journal · 2025Article
- The long and short of it: benchmarking viromics using Illumina, Nanopore and PacBio sequencing technologies.Microbial genomics · 2024Article
- Review
- Article
- The impact of storage buffer and storage conditions on fecal samples for bacteriophage infectivity and metavirome analyses.Microbiome · 2023Article
- Expanding known viral diversity in the healthy infant gut.Nature microbiology · 2023Article
- Molecular characterization of a novel victorivirus (order Ghabrivirales, family Totiviridae) infecting Metarhizium anisopliae.Archives of virology · 2023Article
- An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns.Frontiers in microbiology · 2023Review
- The human gut virome: composition, colonization, interactions, and impacts on human health.Frontiers in microbiology · 2023Review
- Sequencing the SARS-CoV-2 Genome from Stool Samples of Post-acute Cases Implicates a Novel Mutation Associated with Reduced Antibody Neutralization.European journal of biomedical research · 2023Article
- Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome.Microorganisms · 2022Review
- Morphological and Genetic Characterization ofViruses · 2022Article
- Fecal microbiota transfer between young and aged mice reverses hallmarks of the aging gut, eye, and brain.Microbiome · 2022Article
- Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021Review
- Metaviromes Reveal the Dynamics ofViruses · 2021Article
- Analysis of Different Size Fractions Provides a More Complete Perspective of Viral Diversity in a Freshwater Embayment.Applied and environmental microbiology · 2021Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
The human gut microbiome (GM) plays an important role in human health and diseases. However, while substantial progress has been made in understanding the role of bacterial inhabitants of the gut, much less is known regarding the viral component of the GM. Bacteriophages (phages) are viruses attacking specific host bacteria and likely play important roles in shaping the GM. Although metagenomic approaches have led to the discoveries of many new viruses, they remain largely uncultured as their hosts have not been identified, which hampers our understanding of their biological roles. Existing protocols for isolation of viromes generally require relatively high input volumes and are generally more focused on extracting nucleic acids of good quality and purity for down-stream analysis, and less on purifying viruses with infective capacity. In this study, we report the development of an efficient protocol requiring low sample input yielding purified viromes containing phages that are still infective, which also are of sufficient purity for genome sequencing. We validated the method through spiking known phages followed by plaque assays, qPCR, and metagenomic sequencing. The protocol should facilitate the process of culturing novel viruses from the gut as well as large scale studies on gut viromes.
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Registered trials
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