ArticleJournal of biomedical materials research. Part A2019
Application of benzonase in preparation of decellularized lamellar porcine corneal stroma for lamellar keratoplasty.
Article in Journal of biomedical materials research. Part A, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 17 citations in OpenAlex.
- Advances in Corneal Tissue Engineering: Comparative Performance of Bioengineered Grafts in Animal Models.Medicina (Kaunas, Lithuania) · 2025Pooled it
- Cryopreservation preserves the morpho-structural and mechanical integrity of human decellularized tracheas and supports a reduced immunogenic profile.Frontiers in bioengineering and biotechnology · 2026Article
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- A pilot study for decellularizing porcine cornea for future use in corneal regeneration.PloS one · 2025Article
- Advances in preparation of acellular human dermis for tissue banking and transplantation.Cell and tissue banking · 2024Article
- Assessment and validation of enrichment and target capture approaches to improveJournal of clinical microbiology · 2023Article
- Induction of Corneal Epithelial Differentiation of Induced Pluripotent and Orbital Fat-Derived Stem Cells Seeded on Decellularized Human Corneas.Stem cell reviews and reports · 2022Article
- Comparison of porcine corneal decellularization methods and importance of preserving corneal limbus through decellularization.PloS one · 2021Article
- Tissue-Specific Decellularization Methods: Rationale and Strategies to Achieve Regenerative Compounds.International journal of molecular sciences · 2020Review
- Application of benzonase in preparation of decellularized lamellar porcine corneal stroma for lamellar keratoplasty.Journal of biomedical materials research. Part A · 2019Article
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
- Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
This study was to develop anovel and efficient method using endonuclease (benzonase) to preparedecellularized lamellar porcine corneal stroma (DLPCS). The DLPCS was preparedfrom native lamellar porcine corneal stroma (NLPCS) and was treated with 1000 U/ml benzonase for 5hours. We conducted the following measurements and animal transplantation tocompare DLPCS and NLPCS. The residual DNA was decreased significantly from 367.13 ± 19.96 ng/mg to 15.41 ± 0.65 ng/mg after treatment of benzonase by the detection of fluorescentnucleic acid stain. The residual benzonase was also less than detection limit.There was no significant difference in light transmittance of DLPCS comparedwith NLPCS. The extracts of DLPCS did not inhibit cell proliferation of human cornealepithelial cells, mouse fibroblast (L-929) and African green monkey kidney cell(Vero cell). The DLPCS was transplanted into the corneas of rabbit by lamellarkeratoplasty. There was no corneal melting and graft rejection been observedwithin 12 months. The images demonstrated that the repairment of corneal nervesand keratocytes of DLPCS were in indentical shape and reflection compared withnormal cornea, and no obvious inflammatory cells were observed postoperation, byin vivo confocal microscopy. We provided novel evidence that the application ofbenzonase may improve the quality of DLPCS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.