ArticleThe EMBO journal2019
Let-7a-regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor.
Article in The EMBO journal, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 54 citations in OpenAlex.
- The factory enters the fray: how mitochondrial protein trafficking shapes the host response to infection.Journal of virology · 2026Review
- Partners in Silencing: Decoding the Mammalian Argonaute Interactome.Non-coding RNA · 2025Review
- Response to Suresh et al.The EMBO journal · 2025Article
- The dual luciferase assay does not support claims of stop codon readthrough on the AGO1 mRNA.The EMBO journal · 2025Article
- Repression of AGO1 by AGO2 via let-7 microRNAs facilitates embryonic stem cell differentiation.RNA (New York, N.Y.) · 2025Article
- Development of luciferase-based highly sensitive reporters that detect ER-associated protein biogenesis abnormalities.iScience · 2024Article
- Hominini-specific regulation of the cell cycle by stop codon readthrough of FEM1B.Journal of cell science · 2024Article
- DNA Hypomethylation Underlies Epigenetic Swapping betweenEpigenomes · 2024Article
- Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system.EMBO reports · 2024Article
- Ribosome profiling: a powerful tool in oncological research.Biomarker research · 2024Review
- Distal nucleotides affect the rate of stop codon read-through.Quantitative biology (Beijing, China) · 2023Article
- Cellular senescence limits translational readthrough.Biology open · 2021Article
- Influence of virus-host interactions on plant response to abiotic stress.Plant cell reports · 2021Review
- Stop codon readthrough alters the activity of a POU/Oct transcription factor during Drosophila development.BMC biology · 2021Article
- Nonsense suppression therapies in human genetic diseases.Cellular and molecular life sciences : CMLS · 2021Review
- Stop codon read-through of mammalianThe Journal of biological chemistry · 2020Article
- Prevention of dsRNA-induced interferon signaling by AGO1x is linked to breast cancer cell proliferation.The EMBO journal · 2020Article
- Let-7a-regulated translational readthrough of mammalian AGO1 generates a microRNA pathway inhibitor.The EMBO journal · 2019Article
- Argonaute Proteins: From Structure to Function in Development and Pathological Cell Fate Determination.Frontiers in cell and developmental biology · 2019Review
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8 authors at 2 institutions in 2 countries.
Funding
Abstract
Translational readthrough generates proteins with extended C-termini, which often possess distinct properties. Here, we have used various reporter assays to demonstrate translational readthrough of AGO1 mRNA. Analysis of ribosome profiling data and mass spectrometry data provided additional evidence for translational readthrough of AGO1. The endogenous readthrough product, Ago1x, could be detected by a specific antibody both in vitro and in vivo. This readthrough process is directed by a cis sequence downstream of the canonical AGO1 stop codon, which is sufficient to drive readthrough even in a heterologous context. This cis sequence has a let-7a miRNA-binding site, and readthrough is promoted by let-7a miRNA. Interestingly, Ago1x can load miRNAs on target mRNAs without causing post-transcriptional gene silencing, due to its inability to interact with GW182. Because of these properties, Ago1x can serve as a competitive inhibitor of miRNA pathway. In support of this, we observed increased global translation in cells overexpressing Ago1x. Overall, our results reveal a negative feedback loop in the miRNA pathway mediated by the translational readthrough product of AGO1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.