ArticlePloS one2019
Effects of two-dose ceftiofur treatment for metritis on the temporal dynamics of antimicrobial resistance among fecal Escherichia coli in Holstein-Friesian dairy cows.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Long-term effects of systemic ceftiofur and ampicillin on the abundance and duration of shedding of resistant Gram-negative bacteria in the feces of healthy dairy cows: a randomized clinical trial.Frontiers in veterinary science · 2026Article
- Integrated multi-omics analysis reveals that Gongying San ameliorates subclinical mastitis by modulating intestinal microbiota and metabolites in dairy cows.Frontiers in veterinary science · 2025Article
- A cross-sectional comparison of gut metagenomes between dairy workers and community controls.BMC genomics · 2024Article
- Impact of systemic antimicrobial therapy on the faecal microbiome in symptomatic dairy cows.PloS one · 2024Article
- Article
- Antimicrobial drug use and its association with antimicrobial resistance in fecal commensals from cows on California dairies.Frontiers in veterinary science · 2024Article
- Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective.Frontiers in veterinary science · 2024Article
- Two-Dose Ceftiofur Treatment Increases Cephamycinase Gene Quantities and Fecal Microbiome Diversity in Dairy Cows Diagnosed with Metritis.Microorganisms · 2023Article
- Differences in gut metagenomes between dairy workers and community controls: a cross-sectional study.bioRxiv : the preprint server for biology · 2023Article
- Long-Term Effects of Single-Dose Cephalosporin or Macrolide Use on the Prevalence of AmpC and Extended-Spectrum β-Lactamase ProducingMicroorganisms · 2022Article
- Metaphylactic antimicrobial effects on occurrences of antimicrobial resistance in Salmonella enterica, Escherichia coli and Enterococcus spp. measured longitudinally from feedlot arrival to harvest in high-risk beef cattle.Journal of applied microbiology · 2022Article
- Prevalence and Profiles of Antibiotic Resistance GenesMicroorganisms · 2022Article
- Long-read sequencing revealed cooccurrence, host range, and potential mobility of antibiotic resistome in cow feces.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Effect of Antimicrobial Treatment on the Dynamics of Ceftiofur Resistance in Enterobacteriaceae from Adult California Dairy Cows.Microorganisms · 2021Article
- Impact of Ceftiofur Administration in Steers on the Prevalence and Antimicrobial Resistance ofMicroorganisms · 2021Article
- Ceftiofur reduced Fusobacterium leading to uterine microbiota alteration in dairy cows with metritis.Animal microbiome · 2021Article
- A Common Practice of Widespread Antimicrobial Use in Horse Production Promotes Multi-Drug Resistance.Scientific reports · 2020Article
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A pair-matched longitudinal study conducted on three dairy farms in the U.S. High-Plains explored the temporal effects of two-dose ceftiofur crystalline-free acid (CCFA) treatment for metritis on third-generation cephalosporin (3GC) resistance among enteric E. coli in Holstein-Friesian cows. The current 13-day slaughter withholding period does not account for rising populations of third-generation cephalosporin (3GC) resistant bacteria in feces of animals following CCFA treatment. A total of 124 matched-pairs of cows were enrolled in the study. Cows diagnosed with postpartum metritis received the product twice at the labeled dose of 6.6 mg/kg subcutaneously at the base of alternating ears. Untreated cows-absent clinical metritis-were matched on lactation number and calving date. Feces were collected per rectum on days 0 (baseline), 6, 16, 28, and 56. Environmental samples, from watering troughs as well as surface manure from fresh-cow, hospital, maternity, and milking pens, and from the compost pile were collected prior to the animal sample collection period. Historical data on metritis rates and CCFA use were compiled from herd records. On day 0, cows exhibited an overall mean difference of over 4 log10 colony forming units (CFU) comparing 3GC resistant E. coli to the general E. coli population. At the first eligible slaughter date, the difference declined to 3.31 log10 CFU among cows in the CCFA group (P<0.01 compared to control cows). Such differences were no longer observed between the treated and control groups by day 28. Results suggest a 13-day withholding period following the final treatment is insufficient to allow levels of 3GC resistant E. coli to return to baseline. This effect varied by farm and was dependent upon the starting level of resistance. A farm-specific extended slaughter-withholding period could reduce the microbial risk to food products at slaughter.
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