ArticlePloS one2019
Transcriptome analysis of Asparagus officinalis reveals genes involved in the biosynthesis of rutin and protodioscin.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 34 citations in OpenAlex.
- A Comparative Analysis of Nutritional Composition inFood science & nutrition · 2025Article
- Transcriptome analysis reveals candidate genes involved in quercetin biosynthesis in Euphorbia maculata.Scientific reports · 2025Article
- Comparative metabolites profiling of different solvent extracts of Asparagus species cladodes using liquid chromatography-mass spectrometry-based metabolomics and molecular networking.Phytochemical analysis : PCA · 2025Article
- Profiles of phenolics and their synthetic pathways inFood chemistry. Molecular sciences · 2024Article
- Multi-omics identification of a key glycosyl hydrolase gene FtGH1 involved in rutin hydrolysis in Tartary buckwheat (Fagopyrum tataricum).Plant biotechnology journal · 2024Article
- The quality evaluation of 30Food science & nutrition · 2024Article
- Steroidal saponin profiles and their key genes for synthesis and regulation in Asparagus officinalis L. by joint analysis of metabolomics and transcriptomics.BMC plant biology · 2023Article
- Rutin alleviated acrolein-induced cytotoxicity in Caco-2 and GES-1 cells by forming a cyclic hemiacetal product.Frontiers in nutrition · 2022Article
- Metabolomics Reveals Heterogeneity in the Chemical Composition of Green and White Spears of Asparagus (Metabolites · 2021Article
- Identification of key genes associated with secondary metabolites biosynthesis by system network analysis in Valeriana officinalis.Journal of plant research · 2021Article
- Tissue-specific signatures of metabolites and proteins in asparagus roots and exudates.Horticulture research · 2021Article
- Development of an Oriental Medicine Discrimination Method through Analysis of Steroidal Saponins inMolecules (Basel, Switzerland) · 2019Article
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4 authors at 2 institutions in 1 country.
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Abstract
Garden asparagus (Asparagus officinalis L.) is a popular vegetable cultivated worldwide. The secondary metabolites in its shoot are helpful for human health. We analyzed A. officinalis transcriptomes and identified differentially expressed genes (DEGs) involved in the biosynthesis of rutin and protodioscin, which are health-promoting functional compounds, and determined their association with stem color. We sequenced the complete mRNA transcriptome using the Illumina high-throughput sequencing platform in one white, three green, and one purple asparagus cultivars. A gene set was generated by de novo assembly of the transcriptome sequences and annotated using a BLASTx search. To investigate the relationship between the contents of rutin and protodioscin and their gene expression levels, rutin and protodioscin were analyzed using high-performance liquid chromatography. A secondary metabolite analysis using high-performance liquid chromatography showed that the rutin content was higher in green asparagus, while the protodioscin content was higher in white asparagus. We studied the genes associated with the biosynthesis of the rutin and protodioscin. The transcriptomes of the five cultivars generated 336 599 498 high-quality clean reads, which were assembled into 239 873 contigs with an average length of 694 bp, using the Trinity v2.4.0 program. The green and white asparagus cultivars showed 58 932 DEGs. A comparison of rutin and protodioscin biosynthesis genes revealed that 12 of the 57 genes associated with rutin and two of the 50 genes associated with protodioscin showed more than four-fold differences in expression. These DEGs might have caused a variation in the contents of these two metabolites between green and white asparagus. The present study is possibly the first to report transcriptomic gene sets in asparagus. The DEGs putatively involved in rutin and protodioscin biosynthesis might be useful for molecular engineering in asparagus.
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