ArticlePoultry science2019
Performance, intestinal permeability, and gene expression of selected tight junction proteins in broiler chickens fed reduced protein diets supplemented with arginine, glutamine, and glycine subjected to a leaky gut model.
Article in Poultry science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 75 citations in OpenAlex.
- Effects of Dietary Standardized Ileal Digestible Lysine and Amylose/Amylopectin Ratio on Intestinal Morphology, Barrier-Related Gene Expression, and Cecal Microbiota in Broilers Fed Low-Protein Diets.Animals : an open access journal from MDPI · 2026Article
- Functional Amino Acid Supplementation Drives Early Growth and Gut Maturation in Broilers: A Meta-Analysis.Animals : an open access journal from MDPI · 2026Review
- Dietary Amylose/Amylopectin Ratio Modulated Growth Performance, Intestinal Health, and Lipid Metabolism in Goslings Fed Low-Protein Diets.Animals : an open access journal from MDPI · 2026Article
- Architecture, permeability, disruption dynamics and remodeling of tight junction proteins: Implications for performance, health, and welfare of the broiler chicken.Poultry science · 2026Review
- Impact of Selected Natural Bioactive Substances on Immune Response and Tight Junction Proteins in Broiler Chickens.Veterinary medicine and science · 2025Article
- Impact of in ovo and water supplementation of a postbiotic on intestinal integrity and immune responses in broiler chickens challenged with necrotic enteritis.Frontiers in veterinary science · 2025Article
- Growth performance and immune response of broilers during activeAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Nutrient utilisation and growth performance of broiler chickens fed standard or moderately reduced dietary protein diets with and without β-mannanase supplementation.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- The potential of glutamine supplementation in reduced-crude protein diets for chicken-meat production.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Investigation of Potential Gut Health Biomarkers in Broiler Chicks Challenged byPathogens (Basel, Switzerland) · 2024Article
- Article
- Effects ofAnimals : an open access journal from MDPI · 2023Article
- Effects of dietary protein levels on performance, nitrogen excretion, and odor emission of growing pullets and laying hens.Poultry science · 2023Article
- Negative consequences of reduced protein diets supplemented with synthetic amino acids for performance, intestinal barrier function, and caecal microbiota composition of broiler chickens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2023Article
- Goloba kusi (Veterinary world · 2023Article
- Leaky Gut and the Ingredients That Help Treat It: A Review.Molecules (Basel, Switzerland) · 2023Review
- Intra-Amniotic Administration-An Emerging Method to Investigate Necrotizing Enterocolitis, In Vivo (Nutrients · 2022Article
- Dietary 25-Hydroxyvitamin D3 Supplementation Modulates Intestinal Cytokines in Young Broiler Chickens.Frontiers in veterinary science · 2022Article
- Observations supporting hypothetical commensalism and competition between twoFrontiers in microbiology · 2022Article
- Effect of Changes in Photoperiods on Melatonin Expression and Gut Health Parameters in Laying Ducks.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changing dietary protein and amino acids may impact intestinal barrier function. Experiments were conducted in broiler chickens to evaluate supplementation of L-glutamine, glycine, and L-arginine in a reduced protein (RP) diet. Experiment 1 examined the growth performance of broilers fed 5 dietary treatments: 1) a standard diet; 2) an RP diet (193.9 g/kg CP in grower and 176.9 g/kg CP in finisher); 3) RP diet supplemented with 10 g/kg L-Gln; 4) RP diet supplemented with 10 g/kg Gly; 5) RP diet supplemented with 5 g/kg L-Arg. Each experimental diet was replicated 6 times with 10 birds per replicate. In a subset of 96 birds, experiment 2 tested the 4 RP diets with and without dexamethasone (DEX) to induce leaky gut. Each diet was replicated 24 times. Fluorescein isothiocyanate dextran (FITC-d) was used to test intestinal permeability (IP). Gene expression of selected tight junction proteins in ileal and jejunal tissues was assayed by quantitative PCR. From day 7 to 35, the RP diet increased feed intake (FI) (P < 0.05) and body weight gain (BWG) compared with the standard diet while Gln reduced FI and BWG (P < 0.05) compared with RP. Gly had no effect on BWG or FCR. Supplementation of Arg improved FCR from day 21 to 35 and day 7 to 35. In experiment 2, Arg tended to lower FITC-d (P = 0.086). DEX increased passage of FITC-d into the serum (P < 0.001). The villi surface area was increased in birds fed higher Arg (P < 0.05). DEX and diet interacted (P < 0.01) for jejunal claudin-3 mRNA level where DEX upregulated claudin-3 for all diets except the Arg diet. In summary, with a moderate reduction of protein, satisfactory performance can be obtained. Although Gln and Gly had no demonstrable positive effect on IP and performance of broilers, increasing the dietary Arg by approximately 140% improved FCR and showed indications of improved intestinal barrier function of birds fed an RP diet under a stress model.
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