ArticleScience advances2019
Protein lysine de-2-hydroxyisobutyrylation by CobB in prokaryotes.
Article in Science advances, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 76 citations in OpenAlex.
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- Enolase 1: A paradigm of metabolic enzyme moonlighting in tumorigenesis (Review).International journal of oncology · 2026Review
- d-lactate drives lysine d-lactylation to regulate metabolism in Escherichia coli.The Journal of biological chemistry · 2026Article
- Crotonylation driving Streptococcus pneumoniae adaption and virulence.Journal of advanced research · 2026Article
- Deciphering novel enzymatic and non-enzymatic lysine lactylation inEmerging microbes & infections · 2025Article
- Short-chain acyl post-translational modifications in cancers: Mechanisms, roles, and therapeutic implications.Cancer communications (London, England) · 2025Review
- Metabolism-dependent succinylation governs resource allocation for antibiotic resistance.Science advances · 2025Article
- From ketogenic metabolism to targeted therapeutics: current advances in β-hydroxybutyrylation.Frontiers in immunology · 2025Review
- Role of novel protein acylation modifications in sepsis.Frontiers in immunology · 2025Review
- DPF2 reads histone lactylation to drive transcription and tumorigenesis.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- ENL reads histone β-hydroxybutyrylation to modulate gene transcription.Nucleic acids research · 2024Article
- Acetyl-proteome profiling revealed the role of lysine acetylation in erythromycin resistance ofHeliyon · 2024Article
- Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule.Nature communications · 2024Article
- Substrate and Functional Diversity of Protein Lysine Post-translational Modifications.Genomics, proteomics & bioinformatics · 2024Review
- An Integrated Analytical Approach for Screening Functional Post-Translational Modification Sites in Metabolic Enzymes.ACS omega · 2024Article
- A glimpse into novel acylations and their emerging role in regulating cancer metastasis.Cellular and molecular life sciences : CMLS · 2024Review
- Proteome-Wide Analysis of Lysine 2-Hydroxyisobutyrylation in Aspergillus fumigatus.Current microbiology · 2024Article
- Influence of NMolecules (Basel, Switzerland) · 2024Article
- Protein modification by short-chain fatty acid metabolites in sepsis: a comprehensive review.Frontiers in immunology · 2023Review
- Rg3 regulates myocardial pyruvate metabolism via P300-mediated dihydrolipoamide dehydrogenase 2-hydroxyisobutyrylation in TAC-induced cardiac hypertrophy.Cell death & disease · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lysine 2-hydroxyisobutyrylation (Khib) has recently been shown to be an evolutionarily conserved histone mark. Here, we report that CobB serves as a lysine de-2-hydroxyisobutyrylation enzyme that regulates glycolysis and cell growth in prokaryotes. We identified the specific binding of CobB to Khib using a novel self-assembled multivalent photocrosslinking peptide probe and demonstrated that CobB can catalyze lysine de-2-hydroxyisobutyrylation both in vivo and in vitro. R58 of CobB is a critical site for its de-2-hydroxyisobutyrylase activity. Using a quantitative proteomics approach, we identified 99 endogenous substrates that are targeted by CobB for de-2-hydroxyisobutyrylation. We further demonstrated that CobB can regulate the catalytic activities of enolase (ENO) by removing K343hib and K326ac of ENO simultaneously, which account for changes of bacterial growth. In brief, our study dissects a Khib-mediated molecular mechanism that is catalyzed by CobB for the regulation of the activity of metabolic enzymes as well as the cell growth of bacteria.
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