ArticleProceedings of the National Academy of Sciences of the United States of America1988
Identification of cDNA encoding an additional alpha subunit of a human GTP-binding protein: expression of three alpha i subtypes in human tissues and cell lines.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 66 citations in OpenAlex.
- G protein-coupled receptor kinase 3 regulates CCR7 signaling, trafficking, and T cell responses to CCL19 and CCL21.The Journal of biological chemistry · 2026Article
- G-Protein-Coupled Receptor Kinase 2 Limits CCL21-Induced T Cell Migration via Phospholipase Cγ1.Receptors (Basel, Switzerland) · 2025Article
- Early-Life Stress Influences the Transcriptional Activation of Alpha-2A Adrenergic Receptor and Associated Protein Kinase A Signaling Molecules in the Frontal Cortex of Rats.Molecular neurobiology · 2025Article
- AGS3 and GαMolecules (Basel, Switzerland) · 2020Article
- Tubulin, actin and heterotrimeric G proteins: coordination of signaling and structure.Biochimica et biophysica acta · 2014Review
- FTY720 blocks egress of T cells in part by abrogation of their adhesion on the lymph node sinus.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Reciprocal function of Galphai2 and Galphai3 in graft-versus-host disease.European journal of immunology · 2008Article
- Requirement of Galphai in thymic homing and early T cell development.Molecular immunology · 2008Article
- Endogenous expression of adenosine A1, A2 and A3 receptors in rat C6 glioma cells.Neurochemical research · 2007Article
- Inhibition of G alpha i2 activation by G alpha i3 in CXCR3-mediated signaling.The Journal of biological chemistry · 2007Article
- Enhanced expression of GTP-binding proteins in differentiated U937 monocytic cells: possible involvement of tyrosine kinase and protein kinase C.Molecular and cellular biochemistry · 1995Article
- G proteins: critical control points for transmembrane signals.Protein science : a publication of the Protein Society · 1994Review
- Aberrant splicing of Gs alpha transcript in transformed human astroglial and glioblastoma cell lines.Nucleic acids research · 1992Article
- Role of a pertussis toxin sensitive G-protein in mediating the effects of phorbol esters on receptor activated cyclic AMP accumulation in Jurkat cells.Naunyn-Schmiedeberg's archives of pharmacology · 1991Article
- Embryonic stem cells lacking a functional inhibitory G-protein subunit (alpha i2) produced by gene targeting of both alleles.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Heterologously expressed serotonin 1A receptors couple to muscarinic K+ channels in heart.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- A U6 snRNA gene with an internal promoter is juxtaposed to an snRNP protein sequence within an intron of a human G protein gene.Nucleic acids research · 1991Article
- Guanine nucleotide-binding regulatory proteins in retinal pigment epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 1991Article
- Abundance of the alpha-subunits of Gi1, Gi2 and Go in synaptosomal membranes from several regions of the rat brain is increased in hypothyroidism.The Biochemical journal · 1991Article
- Dissection of thymocyte signaling pathways by in vivo expression of pertussis toxin ADP-ribosyltransferase.The EMBO journal · 1990Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The guanine nucleotide-binding proteins (G proteins), which mediate hormonal regulation of many membrane functions, are composed of alpha, beta, and gamma subunits. We have cloned and characterized cDNA from a human T-cell library encoding a form of alpha i that is different from the human alpha i subtypes previously reported [Didsbury, J. R., Ho, Y.-S. & Snyderman, R. (1987) FEBS Lett. 211, 160-164 and Bray, P., Carter, A., Guo, V., Puckett, C., Kamholz, J., Spiegel, A. & Nirenberg, M. (1987) Proc. Natl. Acad. Sci. USA 84, 5115-5119]. alpha i is the alpha subunit of a class of G proteins that inhibits adenylate cyclase and regulates other enzymes and ion channels. This cDNA encodes a polypeptide of 354 amino acids and is assigned to encode the alpha i-3 subtype of G proteins on the basis of its similarity to other alpha i-like cDNAs and the presence of a predicted site for ADP ribosylation by pertussis toxin. We have determined the expression of mRNA for this and two other subtypes of human alpha i (alpha i-1 and alpha i-2) in a variety of human fetal tissues and in human cell lines. All three alpha i subtypes were present in the tissues tested. However, analysis of individual cell types reveals specificity of alpha i-1 expression. mRNA for alpha i-1 is absent in T cells, B cells, and monocytes but is present in other cell lines. The finding of differential expression of alpha i-1 genes may permit characterization of distinct physiological roles for this alpha i subunit. mRNA for alpha i-2 and alpha i-3 was found in all the primary and transformed cell lines tested. Thus, some cells contain all three alpha i subtypes. This observation raises the question of how cells prevent cross talk among receptors that are coupled to effectors through such similar alpha proteins.
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