ArticleMolecular ecology resources2019
Genome-skimming provides accurate quantification for pollen mixtures.
Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Method comparison of microscopy, metabarcoding, and multispectral imaging flow cytometry for identification and relative abundance analysis of insect-dispersed pollen.Scientific reports · 2026Article
- Efficient and scalable training set generation for automated pollen monitoring with Hirst-type samplers.Scientific reports · 2025Article
- A Novel Approach for Pollen Identification and Quantification Using Hybrid Capture-Based DNA Metabarcoding.Ecology and evolution · 2025Article
- Plants, pollinators and their interactions under global ecological change: The role of pollen DNA metabarcoding.Molecular ecology · 2023Article
- Genome skimming as an efficient tool for authenticating commercial products of the pharmaceutically important Paris yunnanensis (Melanthiaceae).BMC plant biology · 2023Article
- Extracting abundance information from DNA-based data.Molecular ecology resources · 2023Review
- Investigating the use of pollen DNA metabarcoding to quantify bee foraging and effects of threshold selection.PloS one · 2023Article
- PollenDetect: An Open-Source Pollen Viability Status Recognition System Based on Deep Learning Neural Networks.International journal of molecular sciences · 2022Article
- Comparing whole-genome shotgun sequencing and DNA metabarcoding approaches for species identification and quantification of pollen species mixtures.Ecology and evolution · 2021Article
- msGBS: A new high-throughput approach to quantify the relative species abundance in root samples of multispecies plant communities.Molecular ecology resources · 2021Article
- DNA barcoding of phytopathogens for disease diagnostics and bio-surveillance.World journal of microbiology & biotechnology · 2021Review
- Testing the effect of pollen exine rupture on metabarcoding with Illumina sequencing.PloS one · 2021Article
- The Treasure Vault Can be Opened: Large-Scale Genome Skimming Works Well Using Herbarium and Silica Gel Dried Material.Plants (Basel, Switzerland) · 2020Article
- Genome-skimming provides accurate quantification for pollen mixtures.Molecular ecology resources · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Studies on foraging partitioning in pollinators can provide critical information to the understanding of food-web niche and pollination functions, thus aiding conservation. Metabarcoding based on PCR amplification and high-throughput sequencing has seen increasing applications in characterizing pollen loads carried by pollinators. However, amplification bias across taxa could lead to unpredictable artefacts in estimation of pollen compositions. We examined the efficacy of a genome-skimming method based on direct shotgun sequencing in quantifying mixed pollen, using mock samples (five and 14 mocks of flower and bee pollen, respectively). The results demonstrated a high level of repeatability and accuracy in identifying pollen from mixtures of varied species ratios. All pollen species were detected in all mocks, and pollen frequencies estimated from the number of sequence reads of each species were significantly correlated with pollen count proportions (linear model, R
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