ArticleJournal of assisted reproduction and genetics2019
Regulation of boar sperm functionality by the nitric oxide synthase/nitric oxide system.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 17 citations in OpenAlex.
- Observation or treatment of paediatric varicoceles: revisiting the evidence.Nature reviews. Urology · 2026Review
- Multi-omics analyses reveal steroid hormone and antioxidant pathways regulating sperm storage capacity in female Muscovy ducks (Cairina moschata).Poultry science · 2026Article
- Reactive Oxygen and Nitrogen Species in Male Reproductive Health: From Molecular Mechanisms to Clinical Consequences.Antioxidants (Basel, Switzerland) · 2026Review
- In Vitro Capacitation in Boar Sperm: Evaluation of Selected Detection Techniques.Methods and protocols · 2026Article
- Medium Composition Determines the Dynamics of Boar In Vitro Sperm Capacitation-Associated Events.International journal of molecular sciences · 2026Article
- Molecular Advances in Male Infertility and Fertility: Importance of Redox Regulation and Oxidative Stress.International journal of molecular sciences · 2026Review
- Time to revise: impact of methodology on boar sperm capacitation in vitro via phosphotyrosine patterns.BMC veterinary research · 2025Review
- Pathogenesis of testicular dysfunction in diabetes: exploring the mechanism and therapeutic interventions.Journal of assisted reproduction and genetics · 2025Review
- Stallion Sperm Freezing with Different Extenders: Role of Antioxidant Activity and Nitric Oxide Production.Animals : an open access journal from MDPI · 2024Article
- Effect of arginine-induced motility and capacitation on RNA population in goat spermatozoa.Veterinary research communications · 2023Article
- Royal-jelly-based apitherapy can attenuate damages to male reproductive parameter following nicotine administration.Animal models and experimental medicine · 2022Article
- The Interaction of NO and HAnimals : an open access journal from MDPI · 2022Article
- Roles of Nitric Oxide in the Regulation of Reproduction: A Review.Frontiers in endocrinology · 2021Review
- The Roles of NO and HInternational journal of molecular sciences · 2020Review
- Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?International journal of molecular sciences · 2020Article
- Molecular Mechanisms Involved in the Impairment of Boar Sperm Motility by Peroxynitrite-Induced Nitrosative Stress.International journal of molecular sciences · 2020Article
- The impact of exogenous testosterone supplementation on spermatogenesis in a rat model of oligoasthenospermia.International journal of clinical and experimental pathology · 2020Article
- Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa.Antioxidants (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
purposeNitric oxide (NO) is a free radical synthesized mainly by nitric oxide synthases (NOSs). NO regulates many aspects in sperm physiology in different species. However, in vitro studies investigating NOS distribution, and how NO influences sperm capacitation and fertilization (IVF) in porcine, have been lacking. Therefore, our study aimed to clarify these aspects.
methodsTwo main experiments were conducted: (i) boar spermatozoa were capacitated in the presence/absence of S-nitrosoglutathione (GSNO), a NO donor, and two NOS inhibitors, N
resultsOur results suggest that NOS distribution could be connected to pathways which lead to capacitation. Treatments showed significant differences after 30 min of incubation, compared to time zero in almost all motility parameters (P < 0.05). When NOSs were inhibited, three protein kinase A (PKA) substrates (~ 75, ~ 55, and ~50 kDa) showed lower phosphorylation levels between treatments (P < 0.05). No differences were observed in total tyrosine phosphorylation levels evaluated by Western blotting nor in situ. The percentage of acrosome-reacted sperm and phosphatidylserine translocation was significantly lower with L-NAME. Both inhibitors reduced sperm intracellular calcium concentration and IVF parameters, but L-NAME impaired sperm ability to penetrate denuded oocytes.
conclusionsThese findings point out to the importance of both sperm and cumulus-oocyte-derived NO in the IVF outcome in porcine.
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Registered trials
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