ArticleExperimental & molecular medicine2019
Efficient exogenous DNA-free reprogramming with suicide gene vectors.
Article in Experimental & molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Induced pluripotent stem cell reprogramming: methodological evolution and challenges in clinical translation.Frontiers in cell and developmental biology · 2026Review
- Article
- Integration-free induced pluripotent stem cells from three endangered Southeast Asian non-human primate species.Scientific reports · 2024Article
- Neuroprotective Effects of Licochalcone D in Oxidative-Stress-Induced Primitive Neural Stem Cells from Parkinson's Disease Patient-Derived iPSCs.Biomedicines · 2023Article
- Reprogramming stem cells in regenerative medicine.Smart medicine · 2022Review
- Intermediate cells of in vitro cellular reprogramming and in vivo tissue regeneration require desmoplakin.Science advances · 2022Article
- Tumorigenicity risk of iPSCsPrecision clinical medicine · 2022Review
- Utilization of Decision Tree Algorithms for Supporting the Prediction of Intensive Care Unit Admission of Myasthenia Gravis: A Machine Learning-Based Approach.Journal of personalized medicine · 2022Article
- Review
- Transitioning into GMP-Compliance: Alternative Methods for Producing Retinal Organoids for Transplantation.Translational vision science & technology · 2021Article
- Generation of Porcine Induced Neural Stem Cells Using the Sendai Virus.Frontiers in veterinary science · 2021Article
- Quantitative evaluation and comparison of two prodrug-activating suicide gene therapies on oral squamous cell carcinoma.American journal of cancer research · 2021Article
- Quantitative Proteomic Analysis of Primitive Neural Stem Cells from LRRK2 G2019S-Associated Parkinson's Disease Patient-Derived iPSCs.Life (Basel, Switzerland) · 2020Article
- Simplified Brain Organoids for Rapid and Robust Modeling of Brain Disease.Frontiers in cell and developmental biology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Reprogramming with episomal vectors is an easy, safe, and cost-effective method to generate exogenous DNA-free (exogene-free) induced pluripotent stem cells (iPSCs). However, the genomic integration of exogenes is observed occasionally. Additionally, the removal of episomal DNA takes more than 70 days in established iPSCs. Here, we inserted the cytosine deaminase (CD) gene from yeast into episomal vectors and used them to reprogram human fibroblasts into iPSCs. These new episomal vectors (CD episomal vectors) were eliminated from the generated iPSCs as early as seven days after 5-fluorocytosine (5-FC) treatment. We also found that cells with the integration of the CD gene perished within two days of 5-FC treatment. In addition, we generated exogene-free induced neural stem cells after one passage by direct reprogramming with CD episomal vectors combined with 5-FC treatment. Conclusively, our novel method allows the rapid and easy isolation of exogene-free reprogrammed cells and can be applied to disease modeling and clinical applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.