ArticleMolecular & cellular proteomics : MCP2019
Delta-S-Cys-Albumin: A Lab Test that Quantifies Cumulative Exposure of Archived Human Blood Plasma and Serum Samples to Thawed Conditions.
Article in Molecular & cellular proteomics : MCP, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 11 citations in OpenAlex.
- An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.Methods (San Diego, Calif.) · 2026Article
- Kinetically Tunable, Subzero-Active, Visual Time-Temperature Indicators Based on the Permanganate-Oxalate Reaction.Reaction chemistry & engineering · 2025Article
- The Permanganate-Oxalate Reaction as a Visual Time-Temperature Indicator: Reaction Systems for Use Above 0 °C.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2025Article
- Delta-S-Cys-Albumin as a Marker of Pediatric Biospecimen Integrity.Biopreservation and biobanking · 2024Article
- Protein "purity," proteoforms, and the albuminome: critical observations on proteome and systems complexity.Frontiers in cell and developmental biology · 2024Article
- Oxidized LDL is stable in human serum under extended thawed-state conditions ranging from -20 °C to room temperature.Journal of mass spectrometry and advances in the clinical lab · 2023Article
- Biochemically Tracked Variability of Blood Plasma Thawed-State Exposure Times in a Multisite Collection Study.Biopreservation and biobanking · 2020Article
- Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay.Journal of visualized experiments : JoVE · 2020Article
- Mass Spectrometry-Based Plasma Proteomics: Considerations from Sample Collection to Achieving Translational Data.Journal of proteome research · 2019Review
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Exposure of blood plasma/serum (P/S) to thawed conditions (> -30 °C) can produce biomolecular changes that skew measurements of biomarkers within archived patient samples, potentially rendering them unfit for molecular analysis. Because freeze-thaw histories are often poorly documented, objective methods for assessing molecular fitness before analysis are needed. We report a 10-μl, dilute-and-shoot, intact-protein mass spectrometric assay of albumin proteoforms called "ΔS-Cys-Albumin" that quantifies cumulative exposure of archived P/S samples to thawed conditions. The relative abundance of S-cysteinylated (oxidized) albumin in P/S increases inexorably but to a maximum value under 100% when samples are exposed to temperatures > -30 °C. The difference in the relative abundance of S-cysteinylated albumin (S-Cys-Alb) before and after an intentional incubation period that drives this proteoform to its maximum level is denoted as ΔS-Cys-Albumin. ΔS-Cys-Albumin in fully expired samples is zero. The range (mean ± 95% CI) observed for ΔS-Cys-Albumin in fresh cardiac patient P/S (
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